Direct Electrochemistry of Methanobactin Functionalized Gold Nanoparticles on Au Electrode
| Autor: | Zhongfan Liu, Zhen-Xing Wang, Yan Wang, Bo-Xin Dou, Jia-Ying Xin, Chungu Xia |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Materials science
010405 organic chemistry Biomedical Engineering Bioengineering General Chemistry Methanobactin 010402 general chemistry Condensed Matter Physics Electrocatalyst Electrochemistry 01 natural sciences 0104 chemical sciences Gold Colloid Electron transfer Colloidal gold Electrode General Materials Science Biosensor Nuclear chemistry |
| Zdroj: | Journal of Nanoscience and Nanotechnology. 18:4805-4813 |
| ISSN: | 1533-4880 |
| DOI: | 10.1166/jnn.2018.15304 |
| Popis: | Mathanobatins (Mb, Mbtins) were immobilized successfully on nanometer-sized gold colloid particles associated with β-mercaptoethylamine. The structures of Mb functionalized gold nanoparticles were characterized and confirmed by UV-vis spectroscopy (UV-vis), FTIR spectra and electrochemical analyses. Direct electron transfer between Mb or copper-loading Mbtins and the modified electrode was investigated without the aid of any electron mediator. The copper-loading Mbtins act as a better electrocatalyst for the reduction of H2O2 than Mb. The copper-loading Mb, with which gold nanoparticles were functionalized, as a model enzyme, was immobilized on gold electrode to construct a novel H2O2 biosensor. In pH 6.4 phosphate buffer solution, the reduction and oxidation peak potentials of Mb functionalized gold nanoparticles modified Au electrode (copper-loading Mbtins) were 0.115 and 0.222 V. On the surface, capacitance per unite area (Cd) of Mb functionalized gold nanoparticles modified electrode were 38 μF cm-2. The immobilized Mb displayed the features of a peroxidase and gave an excellent electrocatalytic response to the reduction of H2O2. The detection limit of Mb functionalized gold nanoparticles (copper-loading) were 09 × 10-5 mA/M (S/N = 3). The Michaelis-Menten constant (Km) was 0.787 mM. Good stability and sensitivity were assessed for the biosensor. |
| Databáze: | OpenAIRE |
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