Identification of candidate biomarkers of the exposure to PCBs in contaminated cattle: A gene expression- and proteomic-based approach
Autor: | F. Dal Piaz, Anna Maria Salzano, Paola Badino, Carlo Nebbia, L. Manzini, Guido Rychen, Giovanni Renzone, Andrea Scaloni, Flavia Girolami, Veronica Spalenza |
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Přispěvatelé: | Universita di Torino, Consiglio Nazionale delle Ricerche (CNR), Università degli Studi di Salerno (UNISA), Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL) |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Proteomics Environmental Engineering Polychlorinated Dibenzodioxins Apolipoprotein B Proteome CYP1B1 [SDV]Life Sciences [q-bio] Gene Expression AhR genes Biomarkers Cattle MALDI-TOF-MS serum profiling Polychlorinated biphenyls TEQ Environmental Chemistry Waste Management and Disposal Pollution Biology Fibrinogen Dioxins Proinflammatory cytokine Microbiology 03 medical and health sciences Blood serum Gene expression medicine Animals [INFO]Computer Science [cs] ComputingMilieux_MISCELLANEOUS 2. Zero hunger Interleukin Polychlorinated Biphenyls Transthyretin 030104 developmental biology 13. Climate action biology.protein Environmental Pollutants medicine.drug Environmental Monitoring |
Zdroj: | Science of the total environment 640-641 (2018): 22–30. doi:10.1016/j.scitotenv.2018.05.284 info:cnr-pdr/source/autori:Girolami F.; Badino P.; Spalenza V.; Manzini L.; Renzone G.; Salzano A.M.; Dal Piaz F.; Scaloni A.; Rychen G.; Nebbia C./titolo:Identification of candidate biomarkers of the exposure to PCBs in contaminated cattle: A gene expression-and proteomic-based approach/doi:10.1016%2Fj.scitotenv.2018.05.284/rivista:Science of the total environment/anno:2018/pagina_da:22/pagina_a:30/intervallo_pagine:22–30/volume:640-641 Science of the Total Environment Science of the Total Environment, Elsevier, 2018, 640-641, pp.22-30. ⟨10.1016/j.scitotenv.2018.05.284⟩ |
ISSN: | 1879-1026 0048-9697 |
Popis: | Dioxins and polychlorinated biphenyls (PCBs) are widespread and persistent contaminants. Through a combined gene expression/proteomic-based approach, candidate biomarkers of the exposure to such environmental pollutants in cattle subjected to a real eco-contamination event were identified. Animals were removed from the polluted area and fed a standard ration for 6 months. The decontamination was monitored by evaluating dioxin and PCB levels in pericaudal fat two weeks after the removal from the contaminated area (day 0) and then bimonthly for six months (days 59, 125 and 188). Gene expression measurements demonstrated that CYP1B1 expression was significantly higher in blood lymphocytes collected in contaminated animals (day 0), and decreased over time during decontamination. mRNA levels of interleukin 2 showed an opposite quantitative trend. MALDI-TOF-MS polypeptide profiling of serum samples ascertained a progressive decrease (from day 0 to 188) of serum levels of fibrinogen β-chain and serpin A3-7-like fragments, apolipoprotein (APO) C-II and serum amyloid A-4 protein, along with an augmented representation of transthyretin isoforms, as well as APOC-III and APOA-II proteins during decontamination. When differentially represented species were combined with serum antioxidant, acute phase and proinflammatory protein levels already ascertained in the same animals (Cigliano et al., 2016), bioinformatics unveiled an interaction network linking together almost all components. This suggests the occurrence of a complex PCB-responsive mechanism associated with animal contamination/decontamination, including a cohort of protein/polypeptide species involved in blood redox homeostasis, inflammation and lipid transport. All together, these results suggest the use in combination of such biomarkers for identifying PCB-contaminated animals, and for monitoring the restoring of their healthy condition following a decontamination process. |
Databáze: | OpenAIRE |
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