Bifidobacterium bifidum and Bacteroides fragilis Induced Differential Immune Regulation of Enteric Glial Cells Subjected to Exogenous Inflammatory Stimulation
| Autor: | Yan-hua Yang, Wei Qian, Xiao-hua Hou, Chi-bing Dai |
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| Rok vydání: | 2022 |
| Předmět: |
Inflammation
Lipopolysaccharides Tumor Necrosis Factor-alpha Interleukin-17 Immunology Toll-Like Receptor 2 Interleukin-10 Bacteroides fragilis Interferon-gamma Humans Interleukin-2 Immunology and Allergy Bifidobacterium bifidum Glial Cell Line-Derived Neurotrophic Factor Interleukin-4 Neuroglia Cells Cultured |
| Zdroj: | Inflammation. 45:2388-2405 |
| ISSN: | 1573-2576 0360-3997 |
| DOI: | 10.1007/s10753-022-01700-6 |
| Popis: | Enteric glial cells (EGCs) are involved in intestinal inflammation. In this study, we will investigate how Bifidobacterium bifidum (B.b.) and Bacteroides fragilis (B.f.) influence EGC regulation. After pretreatment with lipopolysaccharide (LPS) and interferon-γ (IFN-γ), the expressions of major histocompatibility complex class II (MHC-II), CD80, CD86, glial cell line-derived neurotrophic factor (GDNF), toll-like receptor 2 (TLR-2), and tumor necrosis factor-α (TNF-α) in EGCs were detected using polymerase chain reaction and western blot after co-culture with the supernatants of B.b. or B.f. (multiplicity of infection, 40:1 or 80:1). Finally, EGCs were co-cultured with naive CD |
| Databáze: | OpenAIRE |
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