The sea urchin embryo as a model for studying efflux transporters: Roles and energy cost
| Autor: | Rebecca Vega Thurber, Amro Hamdoun, Bryan J. Cole, David Epel |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Embryo
Nonmammalian Cell division Cell Apoptosis Cyclosporins Aquatic Science Vinblastine Oceanography Article biology.animal medicine Animals ATP Binding Cassette Transporter Subfamily B Member 1 Sea urchin Etoposide P-glycoprotein biology Cytotoxins Membrane transport protein Membrane Transport Proteins Embryo Transporter General Medicine Fluoresceins Pollution Cell biology medicine.anatomical_structure Verapamil Biochemistry Sea Urchins Models Animal Quinolines biology.protein Steady state (chemistry) Propionates Energy Metabolism Cell Division |
| Zdroj: | Marine Environmental Research. 62:S1-S4 |
| ISSN: | 0141-1136 |
| Popis: | We describe the use of the sea urchin as a model for studying efflux transporters and estimating energy cost for the cytotoxin protective system provided by these transporters. The unfertilized egg has low transport activity, which increases to a new steady state shortly after fertilization. Activity results from p-glycoprotein (p-gp) and MRP type transporters which protect the embryo from cytotoxic drugs that can disrupt cell division or induce apoptosis. The energy cost is estimated from a novel use of calcein-AM as a substrate; keeping 0.25 microM substrate levels out of the cell utilizes only 0.023% of steady state respiration. |
| Databáze: | OpenAIRE |
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