Human blastocyst outgrowths recapitulate primordial germ cell specification events

Autor: Jasin Taelman, Maria Gomes Fernandes, Petra De Sutter, Susana M. Chuva de Sousa Lopes, Monika Bialecka, Björn Heindryckx, Mina Popovic, Margot Van der Jeught
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Embryology
human embryo quality
MOUSE
human blastocyst outgrowths
SUPPLEMENTATION
Embryo Culture Techniques
lineage specification
0302 clinical medicine
Medicine and Health Sciences
Pseudopodia
Induced pluripotent stem cell
Cells
Cultured
Original Research
education.field_of_study
030219 obstetrics & reproductive medicine
ORIGIN
EMBRYO
Obstetrics and Gynecology
in vitro
Cell Differentiation
Cell biology
peri-implantation development
medicine.anatomical_structure
DIFFERENTIATION
DERIVATION
embryonic structures
Endoderm
Stem cell
STEM-CELLS
human blastocyst
Germ Layers
Pluripotent Stem Cells
Mesoderm
animal structures
in vitro implantation model
Cell Survival
Population
Biology
MASS
03 medical and health sciences
SOX2
Genetics
medicine
Humans
primordial germ cells
Cell Lineage
Blastocyst
implantation model
Embryo Implantation
education
Molecular Biology
outgrowths
Cell Biology
SELF-ORGANIZATION
Embryo
Mammalian
030104 developmental biology
NANOG
Germ Cells
Reproductive Medicine
Epiblast
Octamer Transcription Factor-3
Developmental Biology
Zdroj: Molecular Human Reproduction
MOLECULAR HUMAN REPRODUCTION
ISSN: 1460-2407
Popis: Our current knowledge of the mechanisms leading to human primordial germ cell (PGC) specification stems solely from differentiation experiments starting from human pluripotent stem cells. However, information regarding the origin of PGCs in vivo remains obscure. Here we apply an improved system for extended in vitro culture of human embryos to investigate the presence of PGC-like cells (PGCLCs) 12 days post fertilization (dpf). Good quality blastocysts (n = 141) were plated at 6 dpf and maintained in hypoxia, in medium supplemented with Activin A until 12 dpf. We primarily reveal that 12 dpf outgrowths recapitulate human peri-implantation events and demonstrate that blastocyst quality significantly impacts both embryo viability at 12 dpf, as well as the presence of POU5F1+ cells within viable outgrowths. Moreover, detailed examination of 12 dpf blastocyst outgrowths revealed a population of POU5F1+, SOX2– and SOX17+ cells that may correspond to PGCLCs, alongside POU5F1+ epiblast-like cells and GATA6+ endoderm-like cells. Our findings suggest that, in human, PGC precursors may become specified within the epiblast and migrate either transiently to the extra-embryonic mesoderm or directly to the dorsal part of the yolk sac endoderm around 12 dpf. This is a descriptive analysis and as such the conclusion that POU5F1+ and SOX17+ cells represent bona fide PGCs can only be considered as preliminary. In the future, other PGC markers may be used to further validate the observed cell populations. Overall, our findings provide insights into the origin of the human germline and may serve as a foundation to further unravel the molecular mechanisms governing PGC specification in human.
Databáze: OpenAIRE
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