Mip/LIN-9 Regulates the Expression of B-Myb and the Induction of Cyclin A, Cyclin B, and CDK1
| Autor: | Oscar R. Colamonici, Mark Pilkinton, Julie Song, Raudel Sandoval, Scott A. Ness |
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| Rok vydání: | 2007 |
| Předmět: |
animal structures
Cyclin E Cyclin D Molecular Sequence Data Cyclin A Cyclin B Cell Cycle Proteins Biochemistry Mice Cell Line Tumor CDC2 Protein Kinase Animals Humans Molecular Biology Cyclin Cyclin-dependent kinase 1 Base Sequence biology Tumor Suppressor Proteins fungi Nuclear Proteins Cell Biology Cell cycle Cell biology DNA-Binding Proteins Gene Expression Regulation NIH 3T3 Cells Trans-Activators biology.protein Cyclin A2 |
| Zdroj: | Journal of Biological Chemistry. 282:168-175 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m609924200 |
| Popis: | Members of the novel family of proteins that include Drosophila Mip130, Caenorhabditis elegans LIN-9, and mammalian LIN-9 intervene in different cellular functions such as regulation of transcription, differentiation, transformation, and cell cycle progression. Here we demonstrate that LIN-9, designated as Mip/LIN-9, interacts with B-Myb but not with c-Myb or A-Myb. Mip/LIN-9 regulates the expression of B-Myb in a post-transcriptional manner, and its depletion not only decreases the level of the B-Myb protein but also affects the expression of S phase and mitotic genes (i.e. cyclin A, CDK1, and cyclin B). The critical role of Mip/LIN-9 on the expression of S and G(2)/M genes is further supported by the finding that coexpression of Mip/LIN-9 and B-Myb results in the activation of cyclin A and cyclin B promoter-luciferase reporters, and both proteins are detected on the cyclin A and B promoters. Interestingly, although Mip/LIN-9 promoter occupancy peaks earlier than B-Myb, the highest levels of expression of cyclins A and B correlate with the maximum binding of B-Myb to these promoters. These data support the concept that Mip/LIN-9 is required for the expression of B-Myb, and both proteins collaborate in the control of the cell cycle progression via the regulation of S phase and mitotic cyclins. |
| Databáze: | OpenAIRE |
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