Deacidification of grass silage press juice by continuous production of acetoin from its lactate via an immobilized enzymatic reaction cascade
| Autor: | Volker Sieber, Doris Schieder, Luke W. Guddat, Jörg Carsten, Gerhard Schenk, Dominik Schwarz, Hendrik Hohagen |
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| Přispěvatelé: | Publica |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Environmental Engineering Immobilized enzyme Silage Bioengineering Poaceae 01 natural sciences Continuous production 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology Lactic Acid Waste Management and Disposal Chromatography Renewable Energy Sustainability and the Environment Chemistry Acetoin Substrate (chemistry) General Medicine Biorefinery Lactic acid 030104 developmental biology Titration |
| Zdroj: | Bioresource technology. 245 |
| ISSN: | 1873-2976 |
| Popis: | An immobilized enzymatic reaction cascade was designed and optimized for the deacidification of grass silage press juice (SPJ), thus facilitating the production of bio-based chemicals. The cascade involves a three-step process using four enzymes immobilized in a Ca-alginate gel and uses lactic acid to form acetoin, a value-added product. The reaction is performed with a continuous, pH-dependent substrate feed under oxygenation. With titrated lactic acid yields of up to 91% and reaction times of ca. 6 h was achieved. Using SPJ as titrant yields of 49% were obtained within 6 h. In this deacidification process, with acetoin one value-added bio-based chemical is produced while simultaneously the remaining press juice can be used in applications that require a higher pH. Such, this system can be applied in a multi-product biorefinery concept to take full advantage of nutrient-rich SPJ, which is a widely available and easily storable renewable resource. |
| Databáze: | OpenAIRE |
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