Highly cooperative fluorescence switching of self-assembled squaraine dye at tunable threshold temperatures using thermosensitive nanovesicles for optical sensing and imaging
| Autor: | Li Yan Chan, Chi-Lik Ken Lee, Satoshi Arai, Keitaro Sou |
|---|---|
| Přispěvatelé: | School of Mechanical and Aerospace Engineering, School of Physical and Mathematical Sciences |
| Rok vydání: | 2019 |
| Předmět: |
Materials science
lcsh:Medicine Nanoprobe 02 engineering and technology 010402 general chemistry 01 natural sciences Thermotropic crystal Article Nanomaterials Imaging chemistry.chemical_compound Nanoscience and technology Nano lcsh:Science Lipid bilayer Squaraine dye Thermosensitive Multidisciplinary Quenching (fluorescence) Nanoscale materials business.industry lcsh:R 021001 nanoscience & nanotechnology Fluorescence 0104 chemical sciences Chemistry chemistry Mechanical engineering [Engineering] Optoelectronics lcsh:Q Materials chemistry 0210 nano-technology business |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) |
| ISSN: | 2045-2322 |
| Popis: | Thermosensitive fluorescent dyes can convert thermal signals into optical signals as a molecular nanoprobe. These nanoprobes are playing an increasingly important part in optical temperature sensing and imaging at the nano- and microscale. However, the ability of a fluorescent dye itself has sensitivity and accuracy limitations. Here we present a molecular strategy based on self-assembly to overcome such limitations. We found that thermosensitive nanovesicles composed of lipids and a unique fluorescent dye exhibit fluorescence switching characteristics at a threshold temperature. The switch is rapid and reversible and has a high signal to background ratio (>60), and is also highly sensitive to temperature (10–22%/°C) around the threshold value. Furthermore, the threshold temperature at which fluorescence switching is induced, can be tuned according to the phase transition temperature of the lipid bilayer membrane forming the nanovesicles. Spectroscopic analysis indicated that the fluorescence switching is induced by the aggregation-caused quenching and disaggregation-induced emission of the fluorescent dye in a cooperative response to the thermotropic phase transition of the membrane. This mechanism presents a useful approach for chemical and material design to develop fluorescent nanomaterials with superior fluorescence sensitivity to thermal signals for optical temperature sensing and imaging at the nano- and microscales. |
| Databáze: | OpenAIRE |
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