Circulation of Asian-I and Cosmopolitan genotypes of Dengue-2 virus in northeast India, 2016–2017
Autor: | S Ramachandran, R Kumar, Sasidharanpillai Sabeena, Govindakarnavar Arunkumar, R Bhaskar, Y Bhandari, G D'Souza, A Aithal, S Ujwal, A Jazeel, D Santhosha, Maity Hindol, Amogh Milind Auti |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Adult
Male 0301 basic medicine Serotype Genotype 030106 microbiology 030231 tropical medicine India Dengue virus Biology medicine.disease_cause Virus law.invention Dengue fever lcsh:Infectious and parasitic diseases Dengue Young Adult 03 medical and health sciences 0302 clinical medicine Viral Envelope Proteins law medicine Humans lcsh:RC109-216 Serotyping Phylogeny Polymerase chain reaction Genetic diversity Transmission (medicine) Genetic Variation General Medicine Dengue Virus Middle Aged medicine.disease Virology dengue denv-2 envelope gene genotypes northeast india Cross-Sectional Studies Infectious Diseases RNA Viral Female Parasitology |
Zdroj: | Journal of Vector Borne Diseases, Vol 56, Iss 3, Pp 231-236 (2019) |
ISSN: | 0972-9062 |
Popis: | Background & objectives: Dengue is a major public health problem in northeast India where the majority of the cases go unreported and undiagnosed. Even though all four dengue serotypes are reported, there is a dearth of information on genetic diversity. The present cross-sectional study was undertaken during 2016–17 to determine the genetic variance of dengue virus serotype 2 (DENV-2) based on the envelope (E) glycoprotein gene. Methods: The serum samples collected from the northeast parts of India, as a part of hospital-based acute febrile illness surveillance, were serotyped. Viral RNA was extracted from DENV-2 serum samples using QIAquick® RNA Extraction Kit. The E gene was amplified by conventional reverse-transcriptase polymerase chain reaction (RT-PCR) and the PCR products were sequenced. Results: The E glycoprotein gene of nine serum samples with high viral RNA concentration (Ct |
Databáze: | OpenAIRE |
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