Differential expression of respiratory long-term facilitation among inbred rat strains
| Autor: | Francis J. Golder, Ryan W. Bavis, Jyoti J. Watters, A.Z. Mitchell, Mary Behan, Gordon S. Mitchell, Jenny M Dahlberg, Julia E. R. Wilkerson, Tracy L. Baker-Herman, Peter M. MacFarlane |
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| Rok vydání: | 2009 |
| Předmět: |
Pulmonary and Respiratory Medicine
Male medicine.medical_specialty Physiology Long-Term Potentiation Respiratory System Tropomyosin receptor kinase B Biology Serotonergic Article chemistry.chemical_compound Species Specificity Internal medicine medicine Animals Receptor trkB RNA Messenger Neurotransmitter Hypoxia Phrenic nerve Brain-derived neurotrophic factor General Neuroscience Brain-Derived Neurotrophic Factor Intermittent hypoxia Long-term potentiation Rats Inbred Strains Carbon Dioxide digestive system diseases Rats Inbred F344 Rats Oxygen Phrenic Nerve Endocrinology chemistry Gene Expression Regulation Rats Inbred Lew Anesthesia Receptors Serotonin Serotonin Blood Gas Analysis |
| Zdroj: | Respiratory physiologyneurobiology. 170(3) |
| ISSN: | 1878-1519 |
| Popis: | We tested the hypotheses that: (1) long-term facilitation (LTF) following acute intermittent hypoxia (AIH) varies among three inbred rat strains: Fischer 344 (F344), Brown Norway (BN) and Lewis rats and (2) ventral cervical spinal levels of genes important for phrenic LTF (pLTF) vary in association with pLTF magnitude. Lewis and F344, but not BN rats exhibited significant increases in phrenic and hypoglossal burst amplitude 60min post-AIH that were significantly greater than control experiments without AIH, indicating strain differences in phrenic (98%, 56% and 20%, respectively) and hypoglossal LTF (66%, 77% and 5%, respectively). Ventral spinal 5-HT(2A) receptor mRNA and protein levels were higher in F344 and Lewis versus BN, suggesting that higher 5-HT(2A) receptor levels are associated with greater pLTF. More complex relationships were found for 5-HT(7), BDNF and TrkB mRNA. BN had higher 5-HT(7) and TrkB mRNA versus F344; BN and Lewis had higher BDNF mRNA levels versus F344. Genetic variations in serotonergic function may underlie strain differences in AIH-induced pLTF. |
| Databáze: | OpenAIRE |
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