Expression of fluorescent proteins in Lactobacillus rhamnosus to study host–microbe and microbe–microbe interactions
Autor: | Jos Vanderleyden, Tine Verhoeven, Elke Lievens, Sarah Lebeer, Hans Steenackers, Mariya I. Petrova, Irina Spacova |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Salmonella typhimurium 030106 microbiology Gene Expression Bioengineering Biology Applied Microbiology and Biotechnology Biochemistry Bacterial Adhesion Green fluorescent protein Microbiology law.invention 03 medical and health sciences Probiotic fluids and secretions Lactobacillus rhamnosus law Genes Reporter Lactobacillus Gene expression Humans Research Articles Staining and Labeling Lacticaseibacillus rhamnosus Biofilm food and beverages Epithelial Cells mCherry biology.organism_classification Recombinant Proteins Luminescent Proteins mTagBFP2 adhesion probiotics Biofilms Microbial Interactions Heterologous expression fluorescence Caco-2 Cells Engineering sciences. Technology Biotechnology Research Article |
Zdroj: | Microbial Biotechnology Microbial biotechnology |
ISSN: | 1751-7915 |
Popis: | Probiotic Lactobacillus strains are widely used to benefit human and animal health, although the exact mechanisms behind their interactions with the host and the microbiota are largely unknown. Fluorescent tagging of live probiotic cells is an important tool to unravel their modes of action. In this study, the implementation of different heterologously expressed fluorescent proteins for the labelling of the model probiotic strains Lactobacillus rhamnosusGG (gastrointestinal) and Lactobacillus rhamnosusGR-1 (vaginal) was explored. Heterologous expression of mTagBFP2 and mCherry resulted in long-lasting fluorescence of L. rhamnosusGG and GR-1 cells, using the nisin-controlled expression (NICE) system. These novel fluorescent strains were then used to study in vitro aspects of their microbe-microbe and microbe-host interactions. Lactobacillus rhamnosusGG and L. rhamnosusGR-1 expressing mTagBFP2 and mCherry could be visualized in mixed-species biofilms, where they inhibited biofilm formation by Salmonella Typhimurium-gfpmut3 expressing the green fluorescent protein. Likewise, fluorescent L. rhamnosusGG and L. rhamnosusGR-1 were implemented for the visualization of their adhesion patterns to intestinal epithelial cell cultures. The fluorescent L. rhamnosus strains developed in this study can therefore serve as novel tools for the study of probiotic interactions with their environment. ispartof: Microbial Biotechnology vol:11 issue:2 pages:317-331 ispartof: location:United States status: published |
Databáze: | OpenAIRE |
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