High-dose irradiation in combination with toll-like receptor 9 agonist CpG oligodeoxynucleotide 7909 downregulates PD-L1 expression via the NF-κB signaling pathway in non-small cell lung cancer cells
| Autor: | Tiankui Qiao, Guoxiong Xu, Caixia Gao, Xue Chen, Qi Zhang, Youjun Luo, Xibing Zhuang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
CpG ODN
PD-L1 0301 basic medicine Agonist medicine.drug_class CpG Oligodeoxynucleotide Biology NF-κB 03 medical and health sciences 0302 clinical medicine Gene expression medicine Pharmacology (medical) Receptor non-small cell lung cancer Original Research Gene knockdown irradiation immune escape TLR9 Molecular biology Toll-Like Receptor 9 030104 developmental biology Oncology CpG site 030220 oncology & carcinogenesis |
| Zdroj: | OncoTargets and therapy |
| ISSN: | 1178-6930 |
| DOI: | 10.2147/ott.s116629 |
| Popis: | Objectives Irradiation resistance appears as local recurrence and distant metastasis in advanced stages of non-small cell lung cancer (NSCLC). High-dose irradiation combined with immunotherapy improved overall survival and local control of NSCLC. This study explored the underlying molecular mechanism by which the effect of high-dose irradiation plus toll-like receptor 9 (TLR9) agonist CpG oligodeoxynucleotide (CpG ODN) 7909 on NSCLC. Materials and methods NSCLC H460 cells were exposed to constant high-dose irradiation (6.37 Gy) in irradiation (IR) group and the irradiation plus CpG group. Gene expression was assessed using quantitative reverse transcriptase-polymerase chain reaction and Western blot. Knockdown of nuclear factor kappa B (NF-κB) p65 expression was conducted using p65 siRNA. Results Expression of programmed death-ligand 1 (PD-L1) mRNA was significantly decreased in IR combined with CpG ODN 7909 group compared with the control or IR-alone groups (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|