Preparation monoclonal β-type anti-idiotype antibody of zearalenone and development of green ELISA quantitative detecting technique
| Autor: | Luhuai Shi, Miner Luo, Tao Yu, Hong Wang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
medicine.drug_class Anti-Idiotype Antibody Enzyme-Linked Immunosorbent Assay Food Contamination Monoclonal antibody 01 natural sciences Biochemistry chemistry.chemical_compound Limit of Detection 010608 biotechnology medicine Mycotoxin Zearalenone 010405 organic chemistry Antibodies Monoclonal Green Chemistry Technology General Medicine Mycotoxins Molecular biology Animal Feed 0104 chemical sciences Antibodies Anti-Idiotypic chemistry Monoclonal Biotechnology |
| Zdroj: | Preparative biochemistrybiotechnology. 50(4) |
| ISSN: | 1532-2297 |
| Popis: | Immunoassay has been widely used in the screening of mycotoxins, which may be hazardous to the operator or the environment. This study was to develop a green way to measure zearalenone (ZEN) with a monoclonal β-type anti-idiotype antibody (Ab2β) against ZEN in place of ZEN standard. Six monoclonal β-type anti-idiotype antibodies were prepared. The 50% inhibitory concentration (IC |
| Databáze: | OpenAIRE |
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