Involvement of nitrogen functional groups in high-affinity copper binding in tomato and wheat root apoplasts: spectroscopic and thermodynamic evidence
| Autor: | Patrick Cazevieille, Cédric Garnier, Matthieu Bravin, Stéphanie Guigues, Claire Chevassus-Rosset, Armand Masion, Emmanuel Doelsch |
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| Přispěvatelé: | Centre européen de recherche et d'enseignement des géosciences de l'environnement (CEREGE), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Collège de France (CdF)-Institut national des sciences de l'Univers (INSU - CNRS)-Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA), Processus de Transfert et d'Echanges dans l'Environnement - EA 3819 (PROTEE), Université de Toulon (UTLN), Recyclage et risque (Cirad-Persyst-UPR 78 Recyclage et risque), Département Performances des systèmes de production et de transformation tropicaux (Cirad-PERSYST), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Recyclage et risque (UPR Recyclage et risque), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Collège de France (CdF (institution))-Institut de Recherche pour le Développement (IRD)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Spectroscopie RMN 010501 environmental sciences Ligands Plant Roots 01 natural sciences Biochemistry Oxygen Solanum lycopersicum Cell Wall Organic chemistry Triticum biology U10 - Informatique mathématiques et statistiques Metals and Alloys food and beverages Sorption Nitrogen Physiologie végétale X-Ray Absorption Spectroscopy Membrane Chemistry (miscellaneous) Cuivre Thermodynamics F60 - Physiologie et biochimie végétale Biochimie Relation plante sol Biophysics Triticum aestivum chemistry.chemical_element Biomaterials Cell wall Carbon-13 Magnetic Resonance Spectroscopy Binding site [SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces environment Technique analytique 0105 earth and related environmental sciences Membrane cellulaire Cell Membrane fungi biology.organism_classification Rayon x Copper Plaste chemistry Solanum 010606 plant biology & botany Nuclear chemistry Racine |
| Zdroj: | Metallomics Metallomics, Royal Society of Chemistry, 2016, 8 (3), pp.366-376. ⟨10.1039/c5mt00298b⟩ Metallomics, 2016, 8 (3), pp.366-376. ⟨10.1039/c5mt00298b⟩ |
| ISSN: | 1756-5901 1756-591X |
| DOI: | 10.1039/c5mt00298b⟩ |
| Popis: | International audience; Carboxylic groups located in plant cell walls (CW) are generally considered to be the main copper binding sites in plant roots, despite the presence of other functional groups. The aim of this study was to investigate sites responsible for copper binding in root apoplasts, i.e. CW and outer surface of the plasma membrane (PM) continuum. Binding sites in root apoplasts were investigated by comparing isolated CW of a monocotyledon (Triticum aestivum L.) and dicotyledon (Solanum lycopersicum L.) crop with their respective whole roots. Copper speciation was examined by X-ray absorption (XAS) and C-13-nuclear magnetic resonance spectroscopies while the affinity of ligands involved in copper binding was investigated by modeling copper sorption isotherms. Homogeneous speciation and binding of copper was found in wheat and tomato root apoplasts. Only Cu-N and Cu-O bonds were detected in wheat and tomato root apoplasts. Nitrogen/oxygen ligands were identified in slightly higher proportions (40-70%) than single oxygen ligands. Furthermore, low-and high-affinity binding sites contributed in an almost equivalent proportion to copper binding in root apoplasts. The high-affinity N functional groups embedded in root apoplasts participated in copper binding in the same magnitude than the low-affinity carboxylic groups. |
| Databáze: | OpenAIRE |
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