Kappa-casein gene study with molecular markers in female buffaloes (Bubalus bubalis)
| Autor: | Mario Fernando Cerón Muñoz, Antonio Roberto Otaviano, Janete Aparecida Desidério Sena, Humberto Tonhati |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Veterinary medicine
genomic DNA lcsh:QH426-470 single strand conformation polymorphism polymerase chain reaction Population animal experiment Bubalus jugular vein polymorphism milk yield PCR-RFLP Casein buffaloes Genetics genetic polymorphism Animalia controlled study milk production education Molecular Biology Gene DNA extraction restriction fragment length polymorphism molecular marker buffalo education.field_of_study nonhuman biology cheesemaking allele food and beverages Single-strand conformation polymorphism biology.organism_classification Molecular biology animal genetics Bos taurus SSCP lcsh:Genetics kappa casein Bubalus bubalis female blood sampling Restriction fragment length polymorphism homozygosity Blood sampling |
| Zdroj: | Genetics and Molecular Biology v.28 n.2 2005 Genetics and Molecular Biology Sociedade Brasileira de Genética (SBG) instacron:SBG Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Genetics and Molecular Biology, Volume: 28, Issue: 2, Pages: 237-241, Published: 2005 Genetics and Molecular Biology, Vol 28, Iss 2, Pp 237-241 (2005) |
| Popis: | Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:21:43Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:45:34Z : No. of bitstreams: 1 2-s2.0-30644468865.pdf: 67309 bytes, checksum: fe7a77910d9f485040b4a368a0cc41cd (MD5) Made available in DSpace on 2014-05-27T11:21:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2005-12-01 Caseins comprise make up about 80% of the total protein content of milk and present polymorphism with change in the amino acid sequence. Within this abundance of proteins, kappa-casein is noteworthy, since it has been associated with differences in milk yield, composition and processing. The objective of this study was to observe the existence of polymorphism in the kappa-casein gene in female buffaloes. For this purpose, blood samples from 115 female buffaloes, collected with vacutainer by needle punctionure of the jugular vein, were used. for genomic DNA extraction was done from blood samples. The PCR-RFLP and SSCP techniques demonstrated that the studied animals were monomorphic for the kappa-casein gene. Only allele B was observed in these animals, which was present in homozygosis. Therefore, it was not possible to quantify the gene action on milk yield and its constituents. The monomorphism observed in the population studied would allow the development of a method to identify mixtures of cow and buffalo milk in mozzarella cheese production, especially because, in cattle, the kappa-casein gene is polymorphic. Copyright by the Brazilian Society of Genetics. Universidade Estadual Paulista 'Júlio de Mesquita Filho' Faculdade de Ciências Agrárias e Veterinárias Departamento de Zootecnia, Via de Acesso P.D. Castellane s/n, 14884-900 Jaboticabal, SP Universidade Estadual Paulista 'Júlio de Mesquita Filho' Faculdade de Ciências Agrárias e Veterinárias Departamento de Biologia, Via de Acesso P.D. Castellane s/n, 14884-900 Jaboticabal, SP Universidad de Antioquia Facultad de Ciencias Agrarias, Antioquia Universidade Estadual Paulista 'Júlio de Mesquita Filho' Faculdade de Ciências Agrárias e Veterinárias Departamento de Zootecnia, Via de Acesso P.D. Castellane s/n, 14884-900 Jaboticabal, SP Universidade Estadual Paulista 'Júlio de Mesquita Filho' Faculdade de Ciências Agrárias e Veterinárias Departamento de Biologia, Via de Acesso P.D. Castellane s/n, 14884-900 Jaboticabal, SP |
| Databáze: | OpenAIRE |
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