The potential role of Osteopontin in the maintenance of commensal bacteria homeostasis in the intestine
| Autor: | Yuji Fukushima, Akira Nakajima, Masakazu Hattori, Keiko Sakamoto, Yoko Hamazaki, Koyu Ito, Keiichiro Suzuki, Kouetsu Ogasawara, Nagahiro Minato |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
B Cells lcsh:Medicine Epithelium Pathogenesis Mice 0302 clinical medicine Cellular types Medicine and Health Sciences Cytotoxic T cell Homeostasis Osteopontin lcsh:Science Multidisciplinary Immune cells Genomics Cell biology Intestines medicine.anatomical_structure Medical Microbiology White blood cells Anatomy Research Article Blood cells Immunology Plasma Cells T cells Cytotoxic T cells Mice Transgenic Microbial Genomics Biology Bacterial Physiological Phenomena Microbiology 03 medical and health sciences Immune system stomatognathic system medicine Genetics Animals Antibody-Producing Cells Lamina propria Gene Expression Profiling lcsh:R Biology and Life Sciences Epithelial Cells Gastrointestinal Tract 030104 developmental biology Biological Tissue Animal cells biology.protein Intraepithelial lymphocyte lcsh:Q Microbiome Digestive System 030215 immunology |
| Zdroj: | PLoS ONE, Vol 12, Iss 3, p e0173629 (2017) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Osteopontin (Opn), a multifunctional extracellular matrix protein, is implicated in the pathogenesis of various inflammatory disorders. Under physiologic conditions, its expression is restricted to certain tissues including bone and kidney tubule. However, cellular activation during disease development induces Opn expression in various immune cells. In this study, using Opn-EGFP knock-in (KI) mice we found that CD8α⁺ T cells in the intestinal tissues, including Peyer’s patch, lamina propria and epithelium, express Opn under steady state conditions. Therefore, we examined the role of Opn-expressing CD8α⁺ T cells in intestinal homeostasis. Interestingly, Opn knockout (KO) mice had altered fecal microflora concordant with a reduction of TCRγδ⁺ intraepithelial lymphocytes (IELs). Consistent with this result, both treatment with anti-Opn blocking antibody and deficiency of Opn resulted in decreased survival of TCRγδ⁺ and TCRαβ⁺ IELs. This data suggests that a possibility that Opn may function as a survival factor for IELs in the intestinal tissue. Collectively, these data suggest the possibility that Opn might regulate the homeostasis of intestinal microflora through maintenance of TCRγδ⁺ IELs, possibly by support of IEL survival. |
| Databáze: | OpenAIRE |
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