The serum- and glucocorticoid-induced protein kinase-1 (Sgk-1) mitochondria connection: Identification of the IF-1 inhibitor of the F1F0-ATPase as a mitochondria-specific binding target and the stress-induced mitochondrial localization of endogenous Sgk-1
| Autor: | Anita C. Maiyar, Salvatore Cilia, Gary L. Firestone, Bridget A. O'Keeffe, Maria Vaysberg |
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| Rok vydání: | 2013 |
| Předmět: |
Blotting
Western Fluorescent Antibody Technique Protein Serine-Threonine Kinases Biology Mitochondrion Transfection Biochemistry Mitochondrial apoptosis-induced channel Article Immediate early protein Cell Line Immediate-Early Proteins Mice Stress Physiological Cellular stress response Animals Protein kinase A Proteins General Medicine Mitochondria Cell biology Proton-Translocating ATPases DNAJA3 Mitochondrion localization Signal transduction Signal Transduction |
| Zdroj: | Biochimie. 95:1258-1265 |
| ISSN: | 0300-9084 |
| Popis: | The expression, localization and activity of the serum- and glucocorticoid- induced protein kinase, Sgk-1, are regulated by multiple hormonal and environmental cues including cellular stress. Biochemical fractionation and indirect immunofluorescence demonstrated that sorbitol induced hyperosmotic stress stimulated expression and triggered the localization of endogenous Sgk-1 into the mitochondria of NMuMG mammary epithelial cells. The immunofluorescence pattern of endogenous Sgk-1 was similar to that of a green fluorescent linked fusion protein linked to the N-terminal Sgk-1 fragment that encodes the mitochondrial targeting signal. In the presence or absence of cellular stress, exogenously expressed wild type Sgk-1 efficiently compartmentalized into the mitochondria demonstrating the mitochondrial import machinery per se is not stressed regulated. Co-immunoprecipitation and GST-pull down assays identified the IF-1 mitochondrial matrix inhibitor of the F1F0-ATPase as a new Sgk-1 binding partner, which represents the first observed mitochondrial target of Sgk-1. The Sgk-1/IF-1 interaction requires the 122-176 amino acid region within the catalytic domain of Sgk-1 and is pH dependent, occurring at neutral pH but not at slightly acidic pH, which suggests that this interaction is dependent on mitochondrial integrity. An in vitro protein kinase assay showed that the F1F0-ATPase can be directly phosphorylated by Sgk-1. Taken together, our results suggest that stress-induced Sgk-1 localizes to the mitochondria, which permits access to physiologically appropriate mitochondrial interacting proteins and substrates, such as IF-1 and the F1F0-ATPase, as part of the cellular stressed induced program. |
| Databáze: | OpenAIRE |
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