Isolation and characterization of the orchid cytokinin oxidase DSCKX1 promoter
| Autor: | Hao Yu, Chong-Jin Goh, Shu Hua Yang |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Cytokinins
DNA Plant 5' Flanking Region Physiology Recombinant Fusion Proteins Molecular Sequence Data Arabidopsis Plant Science Biology Gene Expression Regulation Enzymologic chemistry.chemical_compound Gene Expression Regulation Plant Gene expression Gene family Coding region Amino Acid Sequence Orchidaceae Promoter Regions Genetic Gene Glucuronidase Sequence Deletion Base Sequence Sequence Analysis DNA Plants Genetically Modified biology.organism_classification chemistry Biochemistry Regulatory sequence Cytokinin Oxidoreductases Plant Structures |
| Zdroj: | Journal of Experimental Botany. 53:1899-1907 |
| ISSN: | 1460-2431 |
| DOI: | 10.1093/jxb/erf055 |
| Popis: | The orchid DSCKX1 is a new member of the cytokinin oxidase gene family, which catalyses the degradation of cytokinins bearing unsaturated isoprenoid side chains. A 3.7 kb fragment upstream of the DSCKX1 coding region was isolated, sequenced and characterized by deletion analysis of DSCKX1::beta-glucuronidase gene fusions using transient orchid and stable Arabidopsis transformation systems. Functional analysis of 5' deletions defined the 5'-upstream region that directs the expression in distinct tissues. Regulatory elements affecting the cytokinin induction of the DSCKX1 gene have also been delineated |
| Databáze: | OpenAIRE |
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