Beneficial and Safety Properties of a Corynebacterium vitaeruminis Strain Isolated from the Cow Rumen
| Autor: | Nathália Parma Augusto Castilho, Monique Colombo, Svetoslav Dimitrov Todorov, Luís Augusto Nero |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Rumen 030106 microbiology Glycocholic acid Virulence Biology Corynebacterium Microbiology Enterococcus faecalis 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Animals Lactose Molecular Biology Virulence factors Probiotics Corynebacterium vitaeruminis biology.organism_classification Taurocholic acid Listeria monocytogenes Lactobacillus sakei Random Amplified Polymorphic DNA Technique Lactobacillus 030104 developmental biology chemistry Glycodeoxycholic acid Molecular Medicine Cattle Taurodeoxycholic acid Beneficial properties |
| Zdroj: | LOCUS Repositório Institucional da UFV Universidade Federal de Viçosa (UFV) instacron:UFV |
| ISSN: | 1867-1314 |
| Popis: | Corynebacterium vitaeruminis MRU4 was isolated from the cow rumen and was differentiated from other isolates by rep-PCR and RAPD and identified by 16S rRNA sequencing. This strain presented higher survival rates for low pH and bile salts treatments, and it was able to survive and multiply in simulated gastric and intestinal environments. C. vitaeruminis MRU4 had a 53.2% auto-aggregation rate, 42.4% co-aggregation rate with Listeria monocytogenes Scott A, 41.6% co-aggregation rate with Enterococcus faecalis ATCC 19443, 10.0% co-aggregation rate with Lactobacillus sakei ATCC 15521, and 98.2% cell surface hydrophobicity rate. PCR analysis showed the presence of EFTu and map genes. The strain possessed positive results for deconjugation of bile salts (taurocholic acid, taurodeoxycholic acid, glycocholic acid, and glycodeoxycholic acid) and positive results for β-galactosidase activity and lactose assimilation activity (glucose of 8.15 ± 0.01 CFU/ml and lactose of 9.24 ± 0.02 CFU/ml). No virulence was observed by phenotypical tests. C. vitaeruminis MRU4 was resistant to oxacillin, gentamicin, erythromycin, clindamycin, sulfa/trimethoprim, and rifampicin by the disc diffusion method and showed resistance just for vancomycin by the Etest® strips test. The strain was negative for 50 tested virulence and resistance genes based on performed PCR. Based on our knowledge, this is the first report regarding the beneficial potential of one C. vitaeruminis strain. |
| Databáze: | OpenAIRE |
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