Zoledronic Acid Deteriorates Soft and Hard Tissue Healing of Murine Tooth Extraction Sockets in a Dose-Dependent Manner
| Autor: | Haruka Kaneko, Shinichiro Kuroshima, Takashi Sawase, Ryohei Kozutsumi, Muneteru Sasaki, Akira Ishisaki |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Molar
medicine.medical_specialty Pathology Endocrinology Diabetes and Metabolism medicine.medical_treatment Connective tissue Zoledronic Acid Mice Endocrinology Medicine Animals Orthopedics and Sports Medicine Tooth Socket Saline Bone Density Conservation Agents Diphosphonates business.industry X-Ray Microtomography medicine.disease Mice Inbred C57BL medicine.anatomical_structure Zoledronic acid Tooth Extraction Histopathology Bisphosphonate-Associated Osteonecrosis of the Jaw Female Bone marrow business Osteonecrosis of the jaw Wound healing medicine.drug |
| Zdroj: | Calcified tissue international. 110(1) |
| ISSN: | 1432-0827 |
| Popis: | The pathophysiology, histopathology, and immunopathology of bisphosphonate-related osteonecrosis of the jaw (BRONJ) Stage 0 remain unclear. The aim of this study was to investigate the effects of high-dose bisphosphonates on tooth extraction socket healing by creating a murine model of BRONJ Stage 0-like lesions using 8-week-old female C57BL/6J mice. Zoledronic acid (Zol) was administered subcutaneously twice a week for 7 weeks at doses of 0.1 mg/kg/week (moderate dose; Zol-M), 0.5 mg/kg/week (high dose; Zol-H1), and 1.0 mg/kg/week (higher dose; Zol-H2). Saline was used as a control (VC). Both maxillary first molars were extracted 3 weeks after drug treatment. Maxillae, long bones, and sera were collected 4 weeks post-extraction (n = 7 mice/group). Microcomputed tomography, histological, immunohistochemical, and ELISA analyses were performed. A ceiling effect for Zol was noted at the Zol-H1 dose. Osseous healing of extraction sites was significantly impaired with increased necrotic bone and the number of empty lacunae in a Zol dose-dependent manner. Zol significantly decreased epithelial thickness, due to a decrease in thickness of the stratum spinosum, in both Zol-H1 and Zol-H2. Both Zol-H1 and Zol-H2 significantly suppressed the distribution of F4/80+ macrophages in the connective tissue of tooth extraction sockets, although gross healing appeared to be normal. Intriguingly, both Zol-H1 and Zol-H2 significantly increased the numbers of TRAP+ mononuclear cells and detached osteoclasts in the connective tissue and bone marrow of extraction sites compared to VC and Zol-M, correlated with serum TRAcP5b levels. The created murine model of BRONJ Stage 0-like lesions becoming more severe in a dose-dependent manner may help to understand the pathophysiology and histopathology of BRONJ Stage 0 in humans. |
| Databáze: | OpenAIRE |
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