Quantification of mitochondrial DNA damage and copy number in circulating blood of patients with systemic sclerosis by a qPCR-based assay
| Autor: | Kowsar Falahati, Zohreh Jadali, Mitra Ataei, Sara Jafari, Shafieh Movassaghi, Mohammad Hossein Sanati |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Mitochondrial DNA DNA Copy Number Variations Autoimmunity Urine Dermatology medicine.disease_cause DNA Mitochondrial Statistics Nonparametric Scleroderma Real-time polymerase chain reaction Pathogenesis 030207 dermatology & venereal diseases 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Reference Values medicine Humans Electrophoresis Agar Gel Investigation Scleroderma Systemic business.industry Systemic DNA Middle Aged medicine.disease Mitochondrial chemistry Oxidative stress Case-Control Studies 030220 oncology & carcinogenesis RL1-803 Immunology Female Reactive Oxygen Species business DNA Damage |
| Zdroj: | Anais Brasileiros de Dermatologia, Vol 95, Iss 3, Pp 314-319 (2020) Anais Brasileiros de Dermatologia v.95 n.3 2020 Anais brasileiros de dermatologia Sociedade Brasileira de Dermatologia (SBD) instacron:SBD Anais Brasileiros de Dermatologia |
| ISSN: | 0365-0596 |
| Popis: | Background Although not fully understood, oxidative stress has been implicated in the pathogenesis of different autoimmune diseases such as systemic sclerosis. Accumulating evidence indicates that oxidative stress can induce mitochondrial DNA (mtDNA) damage and variations in mtDNA copy number (mtDNAcn). Objective The aim of this study was to explore mtDNAcn and oxidative DNA damage byproducts in peripheral blood of patients with systemic sclerosis and healthy controls. Methods Forty six patients with systemic sclerosis and forty nine healthy subjects were studied. Quantitative real-time PCR used to measure the relative mtDNAcn and the oxidative damage (oxidized purines) of each sample. Results The mean mtDNAcn was lower in patients with systemic sclerosis than in healthy controls whereas the degree of mtDNA damage was significantly higher in cases as compared to controls. Moreover, there was a negative correlation between mtDNAcn and oxidative DNA damage. Study limitations The lack of simultaneous analysis and quantification of DNA oxidative damage markers in serum or urine of patients with systemic sclerosis and healthy controls. Conclusion These data suggest that alteration in mtDNAcn and increased oxidative DNA damage may be involved in the pathogenesis of systemic sclerosis. |
| Databáze: | OpenAIRE |
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