Transcriptomic analysis of Staphylococcus xylosus in the presence of nitrate and nitrite in meat reveals its response to nitrosative stress
| Autor: | Aurore Vermassen, R. Talon, Anne de la Foye, Valentin Loux, Sabine Leroy |
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| Přispěvatelé: | Unité de Microbiologie (MIC), Institut National de la Recherche Agronomique (INRA), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Unité Mathématique Informatique et Génome (MIG), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement |
| Rok vydání: | 2014 |
| Předmět: |
staphylococcus aureus
Microbiology (medical) saucisse viande lcsh:QR1-502 staphylococcus xylosus medicine.disease_cause Microbiology lcsh:Microbiology composé aromatique stress meat 03 medical and health sciences chemistry.chemical_compound Nitrate nitrate medicine Original Research Article starter Nitrite nitrite fermentation Reactive nitrogen species 030304 developmental biology 0303 health sciences biology 030306 microbiology Microbiology and Parasitology Staphylococcus xylosus food and beverages biology.organism_classification nitrosative stress Microbiologie et Parasitologie [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Regulon Biochemistry chemistry Protein repair transcriptome méthionine sulfoxyde Fermentation escherichia coli bactérie gram positif Oxidative stress |
| Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Frontiers Media, 2014, 5, n.p. ⟨10.3389/fmicb.2014.00691⟩ Frontiers in Microbiology (5), n.p.. (2014) Frontiers in Microbiology, Vol 5 (2014) |
| ISSN: | 1664-302X |
| Popis: | International audience; Staphylococcus xylosus is one of the major starter cultures used for meat fermentation because of its crucial role in the reduction of nitrate to nitrite which contributes to color and flavor development. Despite longstanding use of these additives, their impact on the physiology of S. xylosus has not yet been explored. We present the first in situ global gene expression profile of S. xylosus in meat supplemented with nitrate and nitrite at the levels used in the meat industry. More than 600 genes of S. xylosus were differentially expressed at 24 or 72 h of incubation. They represent more than 20% of the total genes and let us to suppose that addition of nitrate and nitrite to meat leads to a global change in gene expression. This profile revealed that S. xylosus is subject to nitrosative stress caused by reactive nitrogen species (RNS) generated from nitrate and nitrite. To overcome this stress, S. xylosus has developed several oxidative stress resistance mechanisms, such as modulation of the expression of several genes involved in iron homeostasis and in antioxidant defense. Most of which belong to the Fur and PerR regulons, respectively. S. xylosus has also counteracted this stress by developing DNA and protein repair. Furthermore, it has adapted its metabolic response carbon and nitrogen metabolism, energy production and cell wall biogenesis to the alterations produced by nitrosative stress. |
| Databáze: | OpenAIRE |
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