A mitochondrial sub-stoichiometric orf87-nad3-nad1 exonA co-transcription unit present in Solanaceae was amplified in the genus Nicotiana
| Autor: | Rosine De Paepe, P. Chetrit, Sophie Gutierres, Christine Lelandais, Fernand Vedel |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
DNA
Plant Transcription Genetic Sequence analysis Molecular Sequence Data Restriction Mapping DNA Mitochondrial Polymerase Chain Reaction Mitochondrial Proteins Open Reading Frames Exon Intergenic region Sequence Homology Nucleic Acid Tobacco Escherichia coli Genetics Amino Acid Sequence Cloning Molecular Gene DNA Primers Plant Proteins Nicotiana Electrophoresis Agar Gel Base Sequence Sequence Homology Amino Acid biology fungi Chromosome Mapping Nucleic Acid Hybridization Exons General Medicine Plants Cosmids biology.organism_classification Mitochondria Blotting Southern Plants Toxic Open reading frame Nicotiana tomentosiformis Nicotiana sylvestris DNA Probes |
| Zdroj: | Current Genetics. 31:55-62 |
| ISSN: | 1432-0983 0172-8083 |
| DOI: | 10.1007/s002940050176 |
| Popis: | Unlike other plant species, two copies of nad3 are present in Nicotiana sylvestris mitochondria. Both are localized downstream from an open reading frame (orf87 ), and are associated with either rps12 or the first exon of the nad1 gene. The orf87-nad3-nad1/A cluster is present in normal stoichiometry in Nicotiana tomentosiformis and is sub-stoichiometric in other Solanaceae, revealing recent amplification in the genus Nicotiana. It is suggested from sequence analysis that this cluster originated in an homologous recombination event that involved the nad3-rps12 intergenic region and the upstream region of an ancestral nad1 gene. Transcription patterns and RT-PCR showed that orf87-nad3-rps12 and orf87-nad3-nad1/A clusters are both co-transcription units. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink