Development of duck hepatitis A virus type 3 vaccine and its use to protect ducklings against infections
| Autor: | Youn-Jeong Lee, Yong-Kuk Kwon, Minjeong Kim, A. Michael Lindberg, HyukMan Kwon, Min-Chul Kim, Jun-Hun Kwon, Seong-Joon Joh |
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| Rok vydání: | 2009 |
| Předmět: |
Duck hepatitis B virus
Virulence Chick Embryo Genome Viral Vaccines Attenuated Virus Hepatitis Virus Duck Microbiology Republic of Korea medicine Animals Phylogeny Poultry Diseases Hepatitis Avihepadnavirus Attenuated vaccine Picornaviridae Infections General Veterinary General Immunology and Microbiology biology Sequence Analysis RNA Public Health Environmental and Occupational Health Hepatitis A Viral Vaccines biology.organism_classification medicine.disease Virology Infectious Diseases Ducks Hepadnaviridae Hepatitis Viral Animal Molecular Medicine RNA Viral |
| Zdroj: | Vaccine. 27(48) |
| ISSN: | 1873-2518 |
| Popis: | A variant type of duck hepatitis A virus (DHAV), DHAV-3 was recently discovered in South Korea and China. Sequence analyses verified that the variant is genetically or serologically different from the DHAV-1 and DHAV-2 types. Duck hepatitis had been reported in South Korea since 1985 and an attenuated DHAV-1 vaccine had efficiently prevented epidemics of DHAV-1 until 2002. Despite the DHAV-1 based vaccine in use the novel DHAV-3 circulating in South Korea remains to be a threat to duckling farming. To develop a live attenuated vaccine against DHAV-3, a representative isolate, AP-04203, was therefore attenuated by repeated passages in SPF chicken embryos 100 times. The 100th passaged virus, AP-04203P100, did not cause clinical sign and mortality in 1-day-old ducklings as well as reversion of virulence capacity. The ducklings vaccinated with AP-04203P100 virus (10(3.0)ELD(50)/0.2ml) on 1-day-old age via the intramuscular injection were well protected from 2 days after challenge with pathogenic AP04203P1 virus via the intramuscular route. In addition, the vaccine candidate also exhibited complete protection against currently circulating pathogenic DHAV-3 isolates. In conclusion, we demonstrate that the live attenuated virus, AP-04203P100, is a promising vaccine candidate facilitating the prevention of duck hepatitis caused by DHAV-3 around East Asia including South Korea. |
| Databáze: | OpenAIRE |
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