Cellular proviral HIV-DNA decline and viral isolation in naïve subjects with <5000 copies/ml of HIV-RNA and >500 × 106/l CD4 cells treated with highly active antiretroviral therapy
| Autor: | Lucia Ercoli, Ercole Concia, M Mannazzu, Giuseppe Tridente, Antonio Aceti, Loredana Sarmati, Giorgio Mancino, Emanuele Nicastri, Giovanni Sotgiu, Marco Trevenzoli, Massimo Andreoni, Saverio Giuseppe Parisi |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Anti-HIV Agents Immunology HIV Infections Indinavir Viremia Biology Virus Proviruses Acquired immunodeficiency syndrome (AIDS) medicine Humans Immunology and Allergy Prospective Studies Sida Reverse Transcriptase Polymerase Chain Reaction virus diseases HIV Protease Inhibitors Middle Aged Provirus medicine.disease biology.organism_classification Virology CD4 Lymphocyte Count Didanosine Stavudine Infectious Diseases Viral replication DNA Viral Lentivirus HIV-1 RNA Viral Reverse Transcriptase Inhibitors Drug Therapy Combination Female Viral disease |
| Zdroj: | AIDS. 14:23-29 |
| ISSN: | 0269-9370 |
| Popis: | To evaluate the decay rate of cellular proviral HIV-DNA and viral replication in patients receiving highly active antiretroviral therapy (HAART) in the very early phase of infection.Thirty-four patients treated with HAART and retrospectively selected for progressive decline of plasma viraemia up to undetectable levels (20 copies/ml), were stratified according to CD4+ cell count and plasma viraemia at base line:500 x 10(6) cells/l with5000 copies/ml (group 1) or with5000 copies/ml (group 2),5000 copies/ml with 300-500 x 10(6) cells/l (group 3) or with300 x 10(6) cells/l (group 4). Plasma HIV-RNA and proviral HIV-DNA were analysed at baseline and after 1, 2, 3, 6, 9 and 12 months of treatment.After 1 year of treatment, a significant decrease of proviral DNA titre was observed in all patients and a decrease1 log was achieved in 24 of 29 subjects of the first three groups. The more pronounced decay of HIV-DNA (half-life 28 weeks) up to50 HIV-DNA copies/10(6) CD4+ cells was detected in patients of group 1. At the year's endpoint, five patients (four in group 1 and one in group 2) had20 HIV-DNA copies. However, HIV strains sensitive to antiretroviral drugs were isolated from peripheral lymphocytes of 16 out of 34 patients.In patients with undetectable plasma viraemia after 1 year of HAART, the highest reduction of proviral DNA up to50 copies/10(6) CD4+ cells was obtained only in subjects in the early asymptomatic phase of infection. Nevertheless, a replication-competent virus can be detected in all phases of antiretroviral therapy. |
| Databáze: | OpenAIRE |
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