Metabolic interaction between anthocyanin and lignin biosynthesis is associated with peroxidase FaPRX27 in strawberry fruit
| Autor: | Jonathan Gershenson, Su-Ying Yeh, Rosario Blanco-Portales, Amparo Monfort, José Luis Caballero, Béatrice Denoyes, Ludwig Ring, Carmen Villatoro, Mathieu Fouché, Stephanie Hücherig, Wilfried Schwab, Thomas Hoffmann, Juan Muñoz-Blanco |
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| Přispěvatelé: | Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM), Universidad de Córdoba [Cordoba], Unité de recherches Espèces Fruitières et Vigne (UREFV), Institut National de la Recherche Agronomique (INRA), Universitat Autònoma de Barcelona (UAB), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Max Planck Institute for Chemical Ecology, Max-Planck-Gesellschaft, European Commission, Ministerio de Ciencia y Tecnología (España), Generalitat de Catalunya, German Research Foundation |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
Physiology [SDV]Life Sciences [q-bio] Plant Science antioxidant capacity Lignin 01 natural sciences Anthocyanins Transcriptome chemistry.chemical_compound biosynthèse de la lignine fragaria x ananassa fraise Oligonucleotide Array Sequence Analysis Plant Proteins 2. Zero hunger anthocyane 0303 health sciences food and beverages Ripening cultivated strawberry Fragaria Biochemistry marqueur microsatellite biopuce expression des gènes Chalcone synthase Molecular Sequence Data Quantitative Trait Loci Color Down-Regulation composé phénolique Biology Quantitative trait locus 03 medical and health sciences Biochemistry and Metabolism pcr Genetics [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Amino Acid Sequence analyse du transcriptome Peroxidase 030304 developmental biology peroxydase Gene Expression Profiling fungi Biosynthetic Pathways Gene expression profiling chemistry Fruit Anthocyanin biology.protein Heterologous expression Sequence Alignment Acyltransferases 010606 plant biology & botany |
| Zdroj: | Plant Physiology 1 (163), 43-60. (2013) Plant Physiology Plant Physiology, American Society of Plant Biologists, 2013, 163 (1), pp.43-60. ⟨10.1104/pp.113.222778⟩ Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0032-0889 1532-2548 |
| Popis: | Plant phenolics have drawn increasing attention due to their potential nutritional benefits. Although the basic reactions of the phenolics biosynthetic pathways in plants have been intensively analyzed, the regulation of their accumulation and flux through the pathway is not that well established. The aim of this study was to use a strawberry (Fragaria × ananassa) microarray to investigate gene expression patterns associated with the accumulation of phenylpropanoids, flavonoids, and anthocyanins in strawberry fruit. An examination of the transcriptome, coupled with metabolite profiling data from different commercial varieties, was undertaken to identify genes whose expression correlated with altered phenolics composition. Seventeen comparative microarray analyses revealed 15 genes that were differentially (more than 200-fold) expressed in phenolics-rich versus phenolics-poor varieties. The results were validated by heterologous expression of the peroxidase FaPRX27 gene, which showed the highest altered expression level (more than 900-fold). The encoded protein was functionally characterized and is assumed to be involved in lignin formation during strawberry fruit ripening. Quantitative trait locus analysis indicated that the genomic region of FaPRX27 is associated with the fruit color trait. Down-regulation of the CHALCONE SYNTHASE gene and concomitant induction of FaPRX27 expression diverted the flux from anthocyanins to lignin. The results highlight the competition of the different phenolics pathways for their common precursors. The list of the 15 candidates provides new genes that are likely to impact polyphenol accumulation in strawberry fruit and could be used to develop molecular markers to select phenolics-rich germplasm. This work was supported by PLANT-KBBE FraGenomics, by the Euroinvestigacion Subprogram from PLANT_KBBE Call, provided by the Spanish Science and Technology Ministry (grant no. EUI2008–03770 to A.M.), by the Centre CONSOLIDER on Agrigenomics and the Xarxa de Referencia en Biotecnología of the Generalitat de Catalunya, and by the Deutsche Forschungsgemeinschaft (grant no. SCHW634/14 to S.-Y.Y. and W.S.). |
| Databáze: | OpenAIRE |
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