Ascorbic acid contribution to ORAC values in berry extracts: An evaluation by the ORAC-pyrogallol red methodology
Autor: | L. Vásquez, Camilo López-Alarcón, Hernán Speisky, E. Lissi, Elias Atala |
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Rok vydání: | 2009 |
Předmět: | |
Zdroj: | FOOD CHEMISTRY Artículos CONICYT CONICYT Chile instacron:CONICYT |
ISSN: | 0308-8146 |
DOI: | 10.1016/j.foodchem.2008.07.063 |
Popis: | An oxygen radical absorbance capacity (ORAC) method based on pyrogallol red bleaching (ORAC-PGR) was used to evaluate the scavenging activity of berry extracts (blackberry, blueberry, and raspberry). Among berry extracts, only raspberry protected pyrogallol red through a clear induction time, related exclusively to ascorbic acid. The lag time allowed an estimation of the ascorbic acid concentration and its contribution to the total ORAC value, estimating that 66% of the ORAC-PGR value of raspberry is related to ascorbic acid. Also, from the induction time, an ascorbic acid concentration of 36 mg per 100 g of fresh weight was estimated for raspberry samples. The ORAC-PGR procedure could be considered as a fast and specific methodology for an estimation of ascorbic acid concentrations in complex samples. |
Databáze: | OpenAIRE |
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