Detection of codon 61 point mutations of the K-ras gene in lung and colorectal cancers by enriched PCR
| Autor: | Takuya Kobatake, Motohiko Tanino, Nobuyoshi Shimizu, Junichi Soh, Shinichi Toyooka, Kazunori Tsukuda, Yasuhiko Inaki, Kazuyasu Kobayashi, Masaaki Yano, Kenji Shimizu, Mamoru Ouchida |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Cancer Research Lung Neoplasms Mutant Mutagenesis (molecular biology technique) Biology medicine.disease_cause Polymerase Chain Reaction medicine Humans Point Mutation Allele Codon Lung cancer Gene Alleles Aged Genetics Mutation Point mutation Cancer DNA Exons General Medicine Middle Aged medicine.disease Molecular biology Genes ras Oncology Female Colorectal Neoplasms Polymorphism Restriction Fragment Length |
| Zdroj: | Oncology Reports. |
| ISSN: | 1791-2431 1021-335X |
| DOI: | 10.3892/or.10.5.1455 |
| Popis: | Mutation of the Kirsten ras (K-ras) gene is one of most common alterations in solid tumors including lung and colorectal cancers. We developed new enriched PCR-RFLP assay to detect mutations of K-ras codon 61 at the 1st and 2nd letters and non-enriched PCR-RFLP assay to detect the 3rd letter mutation. One mutant allele among 10(3) wild-type alleles was detected by enriched PCR-RFLP assay, while one mutant in 10 wild-type alleles was detected by non-enriched PCR-RFLP assay for codon 61 3rd letter. We then examined K-ras codon 12, 13 and 61 mutations in lung and colorectal cancers using these assays. K-ras codon 12 mutation was detected in 10 of 109 (9%) lung cancer and 19 of 83 (23%) colorectal cancer cases. K-ras codon 13 mutation was detected in 2 of 83 (2%) colorectal and 0 of 109 NSCLC cases, respectively. There was no K-ras codon 61 mutation in either type of cancer. Our results demonstrate that enriched PCR-RFLP is a sensitive assay to detect K-ras codon 61 mutation, however, it was extremely rare in lung and colorectal cancers, suggesting organ-specific pathways in mutagenesis of the ras gene family. |
| Databáze: | OpenAIRE |
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