Protective effects of olive oil phenolics oleuropein and hydroxytyrosol against hydrogen peroxide-induced DNA damage in human peripheral lymphocytes
| Autor: | Siniša Tomić, Nataša Zorić, Jadranka Vuković Rodríguez, Nevenka Kopjar, Ivan Kosalec |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Antioxidant
DNA damage medicine.medical_treatment Iridoid Glucosides Pharmaceutical Science antioxidant activity oleuropein hydroxytyrosol olive human lymphocytes comet assay 01 natural sciences Antioxidants 03 medical and health sciences chemistry.chemical_compound Oleuropein medicine Potency Humans Iridoids Food science Lymphocytes Hydrogen peroxide Olive Oil Pharmaceutical industry 030304 developmental biology Pharmacology 0303 health sciences Dose-Response Relationship Drug 010401 analytical chemistry General Medicine Free Radical Scavengers Hydrogen Peroxide Phenylethyl Alcohol In vitro 0104 chemical sciences Comet assay chemistry Hydroxytyrosol dna damage HD9665-9675 |
| Zdroj: | Acta Pharmaceutica, Vol 71, Iss 1, Pp 131-141 (2021) Acta Pharmaceutica Volume 71 Issue 1 |
| ISSN: | 1846-9558 1330-0075 |
| Popis: | This study investigates antioxidant capacity and protective effects of phenolic compounds oleuropein (OLP) and hydroxytyrosol (HT), present in olive oil and olive leaves, against H2O2-induced DNA damage in human peripheral lymphocytes. Antioxidant potency was determined using the measurement of radical-scavenging activity (ABTS∙+ assay), ferric reducing power (FRAP assay) and cupric reducing antioxidant capacity (CUPRAC assay). Both substances were found to be potent antioxidant agents due to their free radical-scavenging activities. Antigenotoxic effects of oleuropein and hydroxytyrosol against H2O2-induced damage in human lymphocytes were evaluated in vitro by alkaline comet assay. At tested concentrations (1, 5, 10 µmol L−1), oleuropein and hydroxytyrosol did not induce a significant increase of primary DNA damage in comparison with the negative control. Pretreatment of human lymphocytes with each of the substances for 120 min produced a dose-dependent reduction of primary DNA damage in the tested cell type. Hydroxytyrosol showed a better protective effect against H2O2-induced DNA breaks than oleuropein which could be associated with their free radical-scavenging efficacy. |
| Databáze: | OpenAIRE |
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