Centromere localization in medaka fish based on half-tetrad analysis
| Autor: | Mitsuru Sakaizumi, Tadashi Sato, Masahiro Furuyama, Haruna Nagaoka |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Genome evolution Oryzias luzonensis Genetic Linkage Centromere Oryzias Interference (genetic) 010603 evolutionary biology 01 natural sciences Polyploidy 03 medical and health sciences Genetics Animals Centromere localization Tetrad Molecular Biology 030304 developmental biology Hybrid 0303 health sciences Genome biology Chromosome Mapping General Medicine biology.organism_classification Triploidy Genetic marker Evolutionary biology Hybridization Genetic Microsatellite Repeats |
| Zdroj: | Genesgenetic systems. 94(4) |
| ISSN: | 1880-5779 |
| Popis: | Gene-centromere (G-C) mapping provides insight into vertebrate genome composition, structure and evolution. Although medaka fish are important experimental animals, no genome-wide G-C map of medaka has been constructed. In this study, we used 112 interspecific triploid hybrids and 152 DNA markers to make G-C maps of all 24 linkage groups (LGs). Under the assumption of 50% interference, 24 centromeres were localized onto all corresponding medaka LGs. Comparison with 21 centromere positions deduced from putative centromeric repeats revealed that 19 were localized inside the centromeric regions of the G-C maps, whereas two were not. Based on the centromere positions indicated in the G-C maps and those of centromeric repeats on each LG, we classified chromosomes as either biarmed or monoarmed; n = 24 = 10 metacentrics/submetacentrics + 14 subtelocentrics/acrocentrics, which is consistent with the results of previous karyological reports. This study helps to elucidate genome evolution mechanisms, and integrates physical and genetic maps with karyological information of medaka. |
| Databáze: | OpenAIRE |
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