Penetration of dynorphin 1–13 across the blood-brain barrier
| Autor: | David S. Baskin, Marsha A. Widmayer, Jeffrey L. Browning, T. D. Turner |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Male
Sucrose endocrine system medicine.medical_specialty Cerebellum Central nervous system Ischemia Hippocampus Neuropeptide Dynorphin Biology Blood–brain barrier Antiviral Agents Dynorphins Rats Sprague-Dawley Cellular and Molecular Neuroscience Aprotinin Bacitracin Endocrinology Leucine Internal medicine medicine Animals Radionuclide Imaging Opioid peptide Chromatography High Pressure Liquid Endocrine and Autonomic Systems General Medicine respiratory system medicine.disease Peptide Fragments Anti-Bacterial Agents Rats Analgesics Opioid Neuroprotective Agents medicine.anatomical_structure nervous system Neurology Blood-Brain Barrier embryonic structures Cats Tyrosine Neuroscience circulatory and respiratory physiology |
| Zdroj: | Neuropeptides. 32:141-149 |
| ISSN: | 0143-4179 |
| DOI: | 10.1016/s0143-4179(98)90029-4 |
| Popis: | Previous studies have demonstrated neuroprotective effects of the opioid peptide dynorphin (dyn) 1-13 in focal cerebral ischemia. The passage of dyn 1-13 across the blood-brain barrier (BBB) was studied by a modification of the Oldendorf technique in the normal rat and cat, as well as in a feline model of experimentally induced focal cerebral ischemia. In the rat, dyn 1-13 penetration of the BBB could not be detected by this technique, even in the presence of peptidase inhibitors. In contrast, dyn 1-13 did cross the BBB into the normal cat hippocampus, cortex and cerebellum. The passage of dyn 1-13 across the BBB was greater in cats with experimentally induced focal cerebral ischemia. Some of the tritium-labeled material which crossed the BBB was confirmed by high performance liquid chromatography to be dyn 1-13. These studies support the hypothesis that the therapeutic effects observed after the peripheral administration of dyn 1-13 to cats with focal cerebral ischemia can be produced by a central mechanism of action. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect