Oribacterium parvum sp. nov. and Oribacterium asaccharolyticum sp. nov., obligately anaerobic bacteria from the human oral cavity, and emended description of the genus Oribacterium
| Autor: | Amanda Hazen, Karen E. Nelson, Sebastian N. Doerfert, Slava S. Epstein, Tine Hohmann, Nicolai Panikov, Manolis Mandalakis, Maria Sizova, William H. Fowle, Paul A. Muller, Ashlee M. Earl, David Stancyk |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
DNA Bacterial New Taxa Molecular Sequence Data Dental Plaque Peptidoglycan Gram-Positive Asporogenous Rods Biology Diaminopimelic Acid Microbiology Bacteria Anaerobic chemistry.chemical_compound RNA Ribosomal 16S Humans Yeast extract Raffinose Phylogeny Ecology Evolution Behavior and Systematics Base Composition Mouth Strain (chemistry) Fatty Acids Nucleic Acid Hybridization Sequence Analysis DNA General Medicine 16S ribosomal RNA Bacterial Typing Techniques Type species chemistry Biochemistry lipids (amino acids peptides and proteins) Female Fermentation Anaerobic bacteria |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 64:2642-2649 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijs.0.060988-0 |
| Popis: | Three strictly anaerobic, Gram-positive, non-spore-forming, rod-shaped, motile bacteria, designated strains ACB1T, ACB7T and ACB8, were isolated from human subgingival dental plaque. All strains required yeast extract for growth. Strains ACB1T and ACB8 were able to grow on glucose, lactose, maltose, maltodextrin and raffinose; strain ACB7T grew weakly on sucrose only. The growth temperature range was 30–42 °C with optimum growth at 37 °C. Major metabolic fermentation end products of strain ACB1T were acetate and lactate; the only product of strains ACB7T and ACB8 was acetate. Major fatty acids of strain ACB1T were C14 : 0, C16 : 0, C16 : 1ω7c dimethyl aldehyde (DMA) and C18 : 1ω7c DMA. Major fatty acids of strain ACB7T were C12 : 0, C14 : 0, C16 : 0, C16 : 1ω7c and C16 : 1ω7c DMA. The hydrolysate of the peptidoglycan contained meso-diaminopimelic acid, indicating peptidoglycan type A1γ. Genomic DNA G+C content varied from 42 to 43.3 % between strains. According to 16S rRNA gene sequence phylogeny, strains ACB1T, ACB8 and ACB7T formed two separate branches within the genus Oribacterium , with 98.1–98.6 % sequence similarity to the type strain of the type species, Oribacterium sinus . Predicted DNA–DNA hybridization values between strains ACB1T, ACB8, ACB7T and O. sinus F0268 were T and ACB8, and strain ACB7T are considered to represent two distinct species of the genus Oribacterium , for which the names Oribacterium parvum sp. nov. and Oribacterium asaccharolyticum sp. nov. are proposed. The type strains are ACB1T ( = DSM 24637T = HM-481T = ATCC BAA-2638T) and ACB7T ( = DSM 24638T = HM-482T = ATCC BAA-2639T), respectively. |
| Databáze: | OpenAIRE |
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