Changes in hepatic metabolic enzyme activities and biliary excretion of 4-nitrophenol in streptozotocin induced diabetic rats
| Autor: | Évora Da Ibéria Leite Nogueira Pinto, Pál Perjési, Zoltán Markovics, Tamás Fischer, Noémi Piroska Kovács, Attila Almási, E. Fischer |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
inorganic chemicals
0301 basic medicine medicine.medical_specialty Sulfotransferase endocrine system diseases medicine.medical_treatment Hepatic excretion Glucuronidation lcsh:RS1-441 lcsh:Pharmacy and materia medica 03 medical and health sciences 0302 clinical medicine Sulfation Sulfate conjugate Internal medicine medicine heterocyclic compounds Experimental diabetes chemistry.chemical_classification biology Chemistry Insulin Streptozotocin enzymes and coenzymes (carbohydrates) 030104 developmental biology Endocrinology Enzyme 4-Nitrophenol Glucuronide conjugate biology.protein Glucuronide 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Brazilian Journal of Pharmaceutical Sciences; Vol. 54 No. 1 (2018); e17347 Brazilian Journal of Pharmaceutical Sciences; Vol. 54 Núm. 1 (2018); e17347 Brazilian Journal of Pharmaceutical Sciences; v. 54 n. 1 (2018); e17347 Brazilian Journal of Pharmaceutical Sciences Universidade de São Paulo (USP) instacron:USP Brazilian Journal of Pharmaceutical Sciences, Vol 54, Iss 1 (2018) Brazilian Journal of Pharmaceutical Sciences, Volume: 54, Issue: 1, Article number: e17347, Published: 07 JUN 2018 |
| ISSN: | 2175-9790 1984-8250 |
| DOI: | 10.1590/s2175-97902018000117347 |
| Popis: | Activity of hepatic metabolic enzymes of glucuronidation and sulfation of 4-nitrophenol (PNP) and biliary excretion of its glucuronide (PNP-G) and sulfate (PNP-S) conjugates have been investigated in control and streptozotocin (STZ)-induced diabetic rats. 500 μM PNP solution was luminally perfused in a cannulated jejunal loop for 90 minutes. It was found that biliary excretion of PNP-G was significantly decreased in the diabetic rats. This effect of STZ could be completely reversed by administration of rapid-acting insulin. Activity of hepatic UDP-glucuronyltransferase and β-glucuronidase was also depressed by the STZ pretreatment. Administration of insulin antagonized the inhibitory action of STZ on UDP-glucuronyltransferase, but the reduced activity of β-glucuronidase was not reversed. Biliary excretion of PNP-S was also depressed in the diabetic rats. Whereas, different effects of insulin administration were observed. Namely, the lower biliary excretion rate of PNP-S was not changed after administration of insulin. Activity of the sulfotransferase and the arylsulfatase enzymes was not altered either by STZ pretreatment or by insulin administration. Biliary excretion of PNP was also significantly depressed by STZ and this depression was not changed after insulin administration. The results call attention to hepatobiliary circulation of low molecular weight xenobiotics and their glucuronide and sulfate conjugates. |
| Databáze: | OpenAIRE |
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