Curcumin reduces apoptosis and promotes osteogenesis of human periodontal ligament stem cells under oxidative stress in vitro and in vivo
| Autor: | Chuanqiang Fu, Yunyi Xie, Le Yu, Huan Chen, Yan Wang, Wei Zhao, Zeyuan Cao, Lingping Tan |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Curcumin Periodontal ligament stem cells Periodontal Ligament Apoptosis medicine.disease_cause 030226 pharmacology & pharmacy General Biochemistry Genetics and Molecular Biology Bone and Bones Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Young Adult 0302 clinical medicine Osteogenesis medicine Periodontal fiber Animals Humans Viability assay General Pharmacology Toxicology and Pharmaceutics Cells Cultured Cell Proliferation Ligaments Regeneration (biology) Stem Cells Cell Differentiation General Medicine Molar Cell biology Transplantation Oxidative Stress 030104 developmental biology chemistry Female Stem cell Oxidative stress Stem Cell Transplantation |
| Zdroj: | Life sciences. 270 |
| ISSN: | 1879-0631 |
| Popis: | Aims Human periodontal ligament stem cells (hPDLSCs) tether the teeth to the surrounding bone and are considered as major functional stem cells responsible for regeneration of the alveolar bone and periodontal ligament tissue. However, the outcome of stem cell regenerative therapy is affected by the survival rate and their differentiation potential of transplanted cells. This is primarily because of local oxidative stress and chronic inflammation at the transplantation site. Therefore, our study aimed to explore whether a natural antioxidant, curcumin could increase the tissue regeneration ability of transplanted hPDLSCs. Main methods A hydrogen peroxide environment and a rat cranial bone defect model were built to mimic the oxidative stress conditions in vitro and in vivo, respectively. We evaluated the effect of curcumin on oxidative status, apoptosis, mitochondrial function and osteogenic differentiation of H2O2-stimulated hPDLSCs in vitro. We also measured the effect of curcumin on cell viability and bone repair ability of transplanted hPDLSCs in vivo. Key findings Our data showed that curcumin enhanced cell proliferation, reduced the reactive oxygen species (ROS) levels and apoptosis, maintained the standard mitochondrial structure and function, and promoted osteogenic differentiation of H2O2-stimulated hPDLSCs. The extracellular regulated protein kinases 1/2 (Erk1/2) signaling pathway was determined to be involved in the osteogenic differentiation of the H2O2-stimulated hPDLSCs. Moreover, curcumin enhanced the viability and the bone repair ability of hPDLSCs in vivo. Significance Curcumin reduced apoptosis and promoted osteogenesis of the hPDLSCs under oxidative stress, and might therefore have a potential clinical use with respect to tissue regeneration. |
| Databáze: | OpenAIRE |
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