Kallikrein gene-modified EPCs induce angiogenesis in rats with ischemic hindlimb and correlate with integrin αvβ3 expression
| Autor: | Yuan Yuan Wang, Jian Rui Li, Qing Da Li, Fu Ji Li, Xiao Meng, Yi Liang Qie, An Bei You, Shen Shen Fu, Yun Zhang, Bao Chuan Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Pathology
medicine.medical_specialty Endothelium Nitric Oxide Synthase Type III Angiogenesis Ischemia lcsh:Medicine Hindlimb Transfection Vasculogenesis medicine Animals Rats Wistar lcsh:Science Integrin alphaVbeta3 Multidisciplinary Neovascularization Pathologic business.industry Stem Cells lcsh:R Kallikrein medicine.disease Rats medicine.anatomical_structure embryonic structures Cancer research cardiovascular system Female Kallikreins lcsh:Q Stem cell business Research Article circulatory and respiratory physiology |
| Zdroj: | PLoS ONE, Vol 8, Iss 9, p e73035 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | BACKGROUND: Human tissue kallikrein (hTK) plays an essential role in the physiological and pathological mechanisms of blood vessels. This study aimed to determine whether angiogenesis induced by endothelial progenitor cells (EPCs) transduced with the adenovirus-mediated hTK gene could improve blood flow in rat hindlimb ischemia in vivo and to establish a promising mechanism in vitro. METHODS: EPCs transduced with adenovirus encoding hTK-162 (i.e., Ad/hTK-transduced EPCs or Ad/GFP-transduced EPCs) were administered to Wister rats with hindlimb ischemia through therapeutic neovascularization. Muscular capillary density (MCD), blood flow (BF), and the number of myofibers were measured at days 7, 14, and 21 after treatment. Expressions of integrin αvβ3 and endothelial nitric oxide synthase (eNOS) were detected on the surface of EPCs. RESULTS: MCD, BF, and the number of myofibers in rats with Ad/hTK-transduced EPCs remarkably increased at day 21 after treatment compared with rats with Ad/GFP-transduced EPCs or the control group (P |
| Databáze: | OpenAIRE |
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