Oligonucleotide-Addressed Covalent 3′-Terminal Derivatization of Small RNA Strands for Enrichment and Visualization
| Autor: | Alexandra Plotnikova, Saulius Klimašauskas, Giedrius Vilkaitis, Aleksandr Osipenko, Milda Nainytė, Viktoras Masevicius |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Small RNA Alkylation Oligonucleotides 010402 general chemistry Methylation 01 natural sciences Article Catalysis Substrate Specificity Synthetic biology 03 medical and health sciences microRNA RNA Small Interfering Oligonucleotide Chemistry Nucleic Acid Heteroduplexes RNA Methyltransferases General Chemistry General Medicine Combinatorial chemistry 0104 chemical sciences MicroRNAs 030104 developmental biology Biochemistry Covalent bond RNA Small Untranslated Biogenesis |
| Zdroj: | Angewandte Chemie. 129:6607-6610 |
| ISSN: | 0044-8249 |
| DOI: | 10.1002/ange.201701448 |
| Popis: | The HEN1 RNA 2'-O-methyltransferase plays important roles in the biogenesis of small non-coding RNAs in plants and proved a valuable tool for selective transfer of functional groups from cofactor analogues onto miRNA and siRNA duplexes in vitro. Herein, we demonstrate the versatile HEN1-mediated methylation and alkylation of small RNA strands in heteroduplexes with a range of complementary synthetic DNA oligonucleotides carrying user-defined moieties such as internal or 3'-terminal extensions or chemical reporter groups. The observed DNA-guided covalent functionalization of RNA broadens our understanding of the substrate specificity of HEN1 and paves the way for the development of novel chemo-enzymatic tools with potential applications in miRNomics, synthetic biology, and nanomedicine. |
| Databáze: | OpenAIRE |
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