DNA-Based Authentication of Botanicals and Plant-Derived Dietary Supplements: Where Have We Been and Where Are We Going?
| Autor: | Michelle R. Lum, David W. Still, Ann M. Hirsch, Denise Fernandes Coutinho Moraes |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Genetic Markers
Quality Control molecular markers DNA Plant Medicinal & Biomolecular Chemistry Pharmaceutical Science Plant Biology Biology Medicinal Analytical Chemistry Ingredient Complementary and Alternative Medicine Drug Discovery botanicals DNA Barcoding Taxonomic Pharmacology Plants Medicinal Traditional medicine business.industry Organic Chemistry Botany Taxonomic High-Throughput Nucleotide Sequencing DNA Plant Pharmacology and Pharmaceutical Sciences Sequence Analysis DNA Plants barcoding Authentication (law) Biotechnology Microscopic observation DNA Barcoding Complementary and alternative medicine Chemical constituents Dietary Supplements Molecular Medicine authentication Identification (biology) Plant Preparations business Drug Contamination Sequence Analysis |
| Zdroj: | Coutinho Moraes, DF; Still, DW; Lum, MR; & Hirsch, AM. (2015). DNA-Based Authentication of Botanicals and Plant-Derived Dietary Supplements: Where Have We Been and Where Are We Going?. Planta Medica, 81(9), 687-695. doi: 10.1055/s-0035-1545843. UCLA: Retrieved from: http://www.escholarship.org/uc/item/6vh492mt Planta medica, vol 81, iss 9 Planta Medica, vol 81, iss 9 |
| ISSN: | 1439-0221 |
| DOI: | 10.1055/s-0035-1545843. |
| Popis: | © Georg Thieme Verlag KG Stuttgart. New York 2015. Herbal medicines and botanicals have long been used as sole or additional medical aids worldwide. Currently, billions of dollars are spent on botanicals and related products, but minimal regulation exists regarding their purity, integrity, and efficacy. Cases of adulteration and contamination have led to severe illness and even death in some cases. Identifying the plant material in botanicals and phytomedicines using organoleptic means or through microscopic observation of plant parts is not trivial, and plants are often misidentified. Recently, DNA-based methods have been applied to these products because DNA is not changed by growth conditions unlike the chemical constituents of many active pharmaceutical agents. In recent years, DNA barcoding methods, which are used to identify species diversity in the Tree of Life, have been also applied to botanicals and plant-derived dietary supplements. In this review, we recount the history of DNA-based methods for identification of botanicals and discuss some of the difficulties in defining a specific bar code or codes to use. In addition, we describe how next generation sequencing technologies have enabled new techniques that can be applied to identifying these products with greater authority and resolution. Lastly, we present case histories where dietary supplements, decoctions, and other products have been shown to contain materials other than the main ingredient stipulated on the label. We conclude that there is a fundamental need for greater quality control in this industry, which if not self-imposed, that may result from legislation. |
| Databáze: | OpenAIRE |
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