Decreased gene expressions of insulin signal molecules in canine hyperadrenocorticism
| Autor: | Yutaka Momota, Saori Shono, Kaori Saeki, Kaori Ueda, Atsuki Murata, Hiroyuki Tazaki, Natsuki Maruyama, Hitomi Oda, Daigo Azakami, Katsumi Ishioka, Akihiro Mori, Ran Akiyama, Satoshi Nozawa, Toshinori Sako |
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| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Adrenocortical Hyperfunction Insulin Receptor Substrate Proteins insulin signal molecules Neutrophils medicine.medical_treatment canine Statistics Nonparametric Insulin resistance Dogs Insulin receptor substrate Internal medicine medicine Internal Medicine Animals Dog Diseases Protein kinase B Protein kinase C Protein Kinase C DNA Primers Regulation of gene expression General Veterinary biology Insulin hyperadrenocorticism Dihydrotestosterone medicine.disease Note Isoenzymes Insulin receptor Endocrinology Gene Expression Regulation biology.protein Female Insulin Resistance Phosphatidylinositol 3-Kinase Proto-Oncogene Proteins c-akt |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 |
| Popis: | Hyperadrenocorticism (HAC) is a common endocrine disorder in dogs, in which excess glucocorticoid causes insulin resistance. Disturbance of insulin action may be caused by multiple factors, including transcriptional modulation of insulin signal molecules which lie downstream of insulin binding to insulin receptors. In this study, gene expressions of insulin signal molecules were examined using neutrophils of the HAC dogs (the untreated dogs and the dogs which had been treated with trilostane). Insulin receptor substrate (IRS)-1, IRS-2, phosphatidylinositol 3-kinase (PI3-K), protein kinase B/Akt kinase (Akt)-2 and protein kinase C (PKC)-lambda were analyzed in the HAC dogs and compared with those from normal dogs. The IRS-1 gene expressions decreased by 37% and 35% of the control dogs in the untreated and treated groups, respectively. The IRS-2 gene expressions decreased by 61% and 72%, the PI3-K gene expressions decreased by 47% and 55%, and the Akt-2 gene expressions decreased by 45% and 56% of the control dogs, similarly. Collectively, gene expressions of insulin signal molecules are suppressed in the HAC dogs, which may partially contribute to the induction of insulin resistance. |
| Databáze: | OpenAIRE |
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