Histochemical investigation of acid mucopolysaccharides in amyloid, colloid and hyaline
| Autor: | Kazuhiko Namba, Yoichiro Sasai, Masakazu Nagata, Kenichiro Inokuchi |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
endocrine system
Amyloid Hyalin Histology Azure A Periodic acid–Schiff stain Lipidoses Skin Diseases Glycosaminoglycan chemistry.chemical_compound Colloid Humans Colloids Hyaline Glycosaminoglycans Skin Chemistry Histocytochemistry Cell Biology General Medicine Amyloidosis Staining Biochemistry Lipoid Proteinosis of Urbach and Wiethe Acid hydrolysis Amyloid (mycology) |
| Zdroj: | Acta histochemica. 62(2) |
| ISSN: | 0065-1281 |
| Popis: | Summary The carbohydrate component in amyloid was histochemically compared with that in colloid or hyaline. Alcian blue, azure A, and periodic acid Schiff were used to stain mucopolysaccharides. In a further attempt to identify various polyanions, staining was carried out with alcian blue containing various concentrations of electrolytes. Methylation, saponification, acid hydrolysis, and digestion with streptomyces or testicular hyaluronidase, or chondroitinase ABC were also employed. The results obtained suggest that the presence of heparitin sulfate in amyloid, of chondroitin sulfate A in hyaline, and of chondroitin sulfate C in colloid. |
| Databáze: | OpenAIRE |
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