Guanosine nucleotides regulate hormone binding of insulin receptors
| Autor: | J G Drachman, Guido Guidotti, E R Mortensen |
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| Rok vydání: | 1992 |
| Předmět: |
Male
medicine.medical_specialty Turkeys medicine.medical_treatment Guanosine Biology Biochemistry chemistry.chemical_compound Internal medicine Insulin receptor substrate medicine Animals Insulin Nucleotide Receptor Molecular Biology chemistry.chemical_classification Cell Membrane Erythrocyte Membrane Temperature Cell Biology Glutathione Guanine Nucleotides Receptor Insulin Rats Insulin receptor Dithiothreitol Endocrinology chemistry Adipose Tissue Solubility Hormone receptor Guanosine 5'-O-(3-Thiotriphosphate) biology.protein Oxidation-Reduction Hormone Research Article |
| Zdroj: | The Biochemical journal. 281 |
| ISSN: | 0264-6021 |
| Popis: | Insulin receptors in turkey erythrocyte and rat adipocyte plasma membranes display non-linear hormone binding by Scatchard analysis. This result is consistent with evidence that the insulin-binding sites are heterogeneous and have at least two affinities for the hormone. Mild reduction of plasma membranes with dithiothreitol, before insulin binding, increased the fraction of hormone binding with high affinity without significantly changing the total number of receptor-binding sites. In the presence of guanosine 5′-[gamma-thio]triphosphate, the amount of receptor with high affinity for insulin in the reduced membranes decreased to that present in the absence of reduction; the effect of the nucleotide was concentration- and temperature-dependent. This decrease in insulin binding was specific for guanine nucleotides. |
| Databáze: | OpenAIRE |
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