Two sulphonated dye compounds which compete for inositol 1,4, 5-trisphosphate binding to rat liver microsomes: effects on 5'-phosphatase activity
| Autor: | Graham F. Pay, Caroline E. Rick, Michael A. Tones, Martin D. Bootman |
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| Rok vydání: | 1990 |
| Předmět: |
Male
Phosphatase Biophysics Receptors Cytoplasmic and Nuclear Receptors Cell Surface Inositol 1 4 5-Trisphosphate Biochemistry chemistry.chemical_compound Sulfation Rosaniline Dyes Animals Inositol 1 4 5-Trisphosphate Receptors Inositol Receptor Inositol phosphate Coloring Agents Molecular Biology chemistry.chemical_classification Molecular mass Triazines Rats Inbred Strains Cell Biology Phosphoric Monoester Hydrolases Rats Kinetics Enzyme chemistry Microsome Microsomes Liver Calcium Channels |
| Zdroj: | Biochemical and biophysical research communications. 166(3) |
| ISSN: | 0006-291X |
| Popis: | The ability of heparin to interact with the Ins 1,4,5-P3 receptor is dependent on its chain length and degree of sulphation. Here we report results obtained with two sulphonated dye compounds of known structures and molecular weights below 1000, cibacron blue and Patent blue. Both compounds compete for Ins 1,4,5-P3 binding to rat liver microsomes and also inhibit Ins 1,4,5-P3 5'-phosphatase activity in the same preparation. Comparison with the effects of heparin show these to be two separate actions of the compounds. |
| Databáze: | OpenAIRE |
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