A novel HPLC method for determination of EDTA in a cataract inhibiting ophthalmic drug
| Autor: | Alireza S. Kord, Irina Tumanova, William L. Matier |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Chromatography
medicine.diagnostic_test Clinical Biochemistry Pharmaceutical Science chemistry.chemical_element High-performance liquid chromatography Copper Dosage form Cataract Analytical Chemistry chemistry.chemical_compound chemistry Nitric acid Spectrophotometry Drug Discovery medicine Edetic Acid Spectrophotometry Ultraviolet Ophthalmic Solutions Acetonitrile Quantitative analysis (chemistry) Spectroscopy Chromatography High Pressure Liquid |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 13(4-5) |
| ISSN: | 0731-7085 |
| Popis: | A novel HPLC method for determination of EDTA in a cataract inhibiting ophthalmic drug product has been developed. In this method EDTA was converted to Cu(II)EDTA complex, using Cu + 2 containing mobile phase, after injection into the chromatographic system. This allowed complexation of EDTA with Cu + 2 without interference from formulation ingredients. The Cu(II)EDTA complex was separated from drug substance, impurities, degradants and other formulation excipients by a 250 x 4.1 mm anion exchange column and detected at UV wavelength 250 nm. The mobile phase consisted of 2 mM cupric nitrate, 11 mM nitric acid, and 25% (v/v) acetonitrile at pH 3.0. This stability indicating assay has been validated and shown to be specific, linear, precise, accurate and rugged for routine EDTA analysis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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