Mitochondrial enzymes in hereditary ataxias
| Autor: | Joseph DeCicco, John P. Blass, Jesse M. Cedarbaum, Kwan-Fu Rex Sheu, Bradford J. Harding, Young Tai Kim |
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| Rok vydání: | 1988 |
| Předmět: |
Blood Platelets
medicine.medical_specialty Ataxia Immunocytochemistry Pyruvate Dehydrogenase Complex Biology Mitochondrion Biochemistry Antibodies Cellular and Molecular Neuroscience Glutamate Dehydrogenase Internal medicine medicine Humans Platelet Cells Cultured chemistry.chemical_classification Immunochemistry Glutamate dehydrogenase Fibroblasts Pyruvate dehydrogenase complex Pathophysiology Mitochondria Enzyme Endocrinology chemistry Neurology (clinical) medicine.symptom |
| Zdroj: | Metabolic Brain Disease. 3:151-160 |
| ISSN: | 1573-7365 0885-7490 |
| DOI: | 10.1007/bf01001015 |
| Popis: | As a test of the hypothesis that mitochondrial abnormalities are common in patients with hereditary ataxias, the activities of two mitochondrial enzymes were studied in platelets from an unselected series of patients. For the group of ataxics, the activity of the pyruvate dehydrogenase complex (PDHC) was 68% of the control (P less than 0.01) and that of glutamate dehydrogenase (GDH) was 81% of the control (P less than 0.05). Of the ataxics studied, 30% had activities of either or both mitochondrial enzymes more than 2 SD below the control mean. Immunoblots of PDHC revealed antibody cross-reacting material in platelets and fibroblasts very similar to those in human brain and appeared normal in platelets from patients with ataxias. Immunoblots of GDH showed a single antibody cross-reacting material in brain but at least two species in normal fibroblasts and platelets. The pathophysiology of hereditary ataxias may often involve mitochondrial damage associated with secondary decreases in the activities of mitochondrial enzymes. |
| Databáze: | OpenAIRE |
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