| Popis: |
Objective The aim of this study was to investigate the effects of rottlerin on growth inhibition as well as autophagy in EJ cells in vitro. Methods Cell viability, measured by MTT assay, apoptosis of cells was stained by annexin V-FITC/PI staining. Results Cell viability, measured by MTT assay, was suppressed in a dose-dependent and time-dependent manner. Moreover, apoptosis of cells was significantly increased with the treatment of rottlerin as indicated by increased annexin V-FITC/PI staining and cell cycle distribution being blocked in G1 phases. Rottlerin treatment also enhanced activation of autophagy with increased expression of LC3-II and appearance of autophagosome. Conclusions The increased level of protein LC3-II and autophagosome suggests that autophagy may contribute to apoptosis. In addition, Pro caspase-3, cleaved caspase-3, total PARP, and cleaved-PARP showed no apparent changes in cells treated with rottlerin, which indicates that caspases may not play a key role during the process of apoptosis induced by rottlerin. |
