Tissue source dictates lineage outcome of human fetal CD34(+)CD38(-) cells
| Autor: | David T. Scadden, Anita H. Piascik, Gregor B. Adams, Richard H. Evans, Tao Cheng, Mark C. Poznansky, Ivona T. Olszak, Russell B. Foxall |
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| Rok vydání: | 2001 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Myeloid T-Lymphocytes CD34 Cell Culture Techniques Antigens CD34 Cell Separation Thymus Gland Biology Immunophenotyping Mice Fetus NAD+ Nucleosidase Antigens CD Genetics medicine Animals Humans ADP-ribosyl Cyclase Molecular Biology Cells Cultured Membrane Glycoproteins Hematopoietic Tissue Cell Biology Hematology Hematopoietic Stem Cells ADP-ribosyl Cyclase 1 Antigens Differentiation Cell biology Hematopoiesis Mice Inbred C57BL Haematopoiesis medicine.anatomical_structure Liver Organ Specificity Bone marrow Stem cell Ex vivo Spleen Multipotentiality |
| Zdroj: | Experimental hematology. 29(6) |
| ISSN: | 0301-472X |
| Popis: | Objective The translocation from fetal liver hematopoiesis to secondary organs occurs during the second trimester of human gestation. It has been hypothesized that stem cells migrate and acquire lineage potential based on cues specific to the adopted microenvironment. We evaluated primitive hematopoietic cell populations in the fetal human to determine if lineage restriction precedes or follows translocation to sites of hematopoietic activity including thymus, spleen, bone marrow, and liver. Methods Sets of hematopoietic tissues from individual second-trimester human abortuses were used to compare and quantitate the lineage outcome of immunophenotypically primitive cells from each of the hematopoietic organs using ex vivo myeloid and lymphoid differentiation systems. Results Despite uniformity in immunophenotype, functional capabilities were highly restricted by the tissue of origin and alteration in the ex vivo differentiation context did not lead to a change in differentiation outcome. Conclusion Translocation of primitive cells from fetal liver to tissues of mature hematopoietic activity is associated with tissue-specific, quantitative changes in differentiation potential that are unresponsive to alternative differentiation environments. These data suggest that multipotentiality is lost prior to or upon stem-cell migration in the developing human. It is not persistent with residence in a secondary hematopoietic organ. |
| Databáze: | OpenAIRE |
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