Flexibility of A · Pairs in Left-Handed DNA Helices
| Autor: | Jaroslav Šponar, Jaromir Vlach |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Physics
Base Composition Flexibility (anatomy) Base Sequence Molecular Sequence Data Phase (waves) DNA General Medicine Left-Handed DNA Out of phase Crystallography medicine.anatomical_structure Cruciform Structural Biology medicine Nucleic Acid Conformation Thermodynamics Alternation (formal language theory) Base sequence Molecular Biology |
| Zdroj: | Journal of Biomolecular Structure and Dynamics. 7:1083-1093 |
| ISSN: | 1538-0254 0739-1102 |
| DOI: | 10.1080/07391102.1990.10508548 |
| Popis: | We have analysed by various approaches the structure of cloned synthetic sequences in supercoiled plasmids. Individual inserts were formed by d(C-G)n blocks interrupted by the presence of A.T pairs positioned either in phase or out of phase of pur-pyr alternation. Based on the thermodynamic analysis we obtained results confirming that A.T pairs are easily incorporated into left-handed helices without significant energetic penalty. Sequences GTAC which are known to form cruciform structures in multiple repetition underwent a B-Z transition. In the case of plasmids containing AA/TT code words and substantial discontinuities in purine-pyrimidine alternation our analysis indicates that Z-Z junctions formed by A.T pairs contributed little to the overall energetic demands of the B-Z transition probably thanks to their high conformational flexibility. |
| Databáze: | OpenAIRE |
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