Human Beta Defensin 3 induces maturation of human langerhans cell like dendritic cells: An antimicrobial peptide that functions as an endogenous adjuvant
| Autor: | Adriana T. Larregina, Eric Fluharty, Yvonne K. Mburu, Alicia R. Mathers, Robert L. Ferris, Louis D. Falo, Laura K. Ferris |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Langerhans cell
beta-Defensins MAP Kinase Signaling System T-Lymphocytes Antimicrobial peptides Human skin chemical and pharmacologic phenomena Dermatology Biology GTP-Binding Protein alpha Subunits Gi-Go Biochemistry Article Immunophenotyping 03 medical and health sciences Interferon-gamma 0302 clinical medicine Immune system Adjuvants Immunologic Cell Movement medicine Humans Molecular Biology 030304 developmental biology Cell Proliferation 0303 health sciences Innate immune system integumentary system Chemokine CCL21 CCL19 NF-kappa B Cell Polarity Cell Biology Acquired immune system 3. Good health Cell biology Beta defensin medicine.anatomical_structure Langerhans Cells Immunology Skin-derived dendritic cells Myeloid Differentiation Factor 88 Human beta-defensin 3 Chemokine CCL19 Immunization 030215 immunology Antimicrobial Cationic Peptides |
| Zdroj: | The Journal of investigative dermatology |
| ISSN: | 1523-1747 0022-202X |
| Popis: | Human beta-defensins (hBDs) are antimicrobial peptides that have an important role in innate immune responses at epithelial barriers such as the skin. However, the role that hBDs have in initiating cellular immune responses that contribute to antigen-specific adaptive immunity is not well understood. Here we show that one member of the hBD family, hBD3, can induce maturation and T-helper type 1 skewing function in human Langerhans cell–like dendritic cells (LC-DCs). Specifically, hBD3 potently induces phenotypic maturation of LC-DCs, including increased expression of CCR7, which mediates functional chemotactic responses to CCL19 and CCL21. hBD3-stimulated LC-DCs induce strong proliferation of and IFN-γ secretion by naive human T cells. hBD3 also induces phenotypic maturation of primary human skin–migratory DCs derived from human skin explants. These results suggest an important role for hBD3 in inducing DC activation, migration, and polarization. Thus, hBD3 contributes to the integration of innate and adaptive immune responses in the skin, and may be a useful adjuvant for skin immunization and an important factor in the pathophysiology of inflammatory skin diseases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|