Exposure to pesticides and heterozygote genotype of GSTP1-Alw26I are associated to Parkinson's disease

Autor: Fabio de Nazare Oliveira, Denise P. Martins, Camila M. Mazeti, Gabriela S. Longo, Marcelo Arruda Nakazone, Michele Lima Gregório, Gisele S. Amorim, Waldir Antonio Tognola, Dorotéia Rossi Silva Souza, Greiciane Silva Florim, Marcela Augusta de Souza Pinhel, Caroline L. Sado
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Male
Heterozygote
Site-Specific DNA-Methyltransferase (Adenine-Specific)
Parkinson's disease
DNA-Cytosine Methylases
Physiology
polimorfismo genético
xenobioticos
Polymerase Chain Reaction
lcsh:RC321-571
GSTP1
Sex Factors
Gene Frequency
Polymorphism (computer science)
Risk Factors
Genotype
glutathione transferase
Medicine
Humans
genetic polymorphism
xenobiotics
glutationa transferase
polimorfismo genetico
Parkinson Disease
Secondary
Pesticides
Allele frequency
lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry
Aged
Genetics
Aged
80 and over
Polymorphism
Genetic
business.industry
Case-control study
Heterozygote advantage
Middle Aged
medicine.disease
doença de Parkinson
Parkinson disease
xenobióticos
Neurology
Glutathione S-Transferase pi
Case-Control Studies
doenca de Parkinson
Female
Neurology (clinical)
Restriction fragment length polymorphism
business
Zdroj: Arquivos de Neuro-Psiquiatria, Volume: 71, Issue: 7, Pages: 446-452, Published: JUL 2013
Arquivos de Neuro-Psiquiatria v.71 n.7 2013
Arquivos de neuro-psiquiatria
Academia Brasileira de Neurologia
instacron:ABNEURO
Arquivos de Neuro-Psiquiatria, Vol 71, Iss 7, Pp 446-452 (2013)
Popis: ObjectiveThis study aimed to analyze the frequency of GSTP1-Alw26I polymorphism and to estimate its association with toxic substances in Parkinson's disease (PD).MethodsA study group with 154 patients - subdivided into familial and sporadic PD groups - and 158 elderly individuals without the disease (control group) were evaluated. GSTP1-Alw26I polymorphism was analyzed by polymerase chain reaction/restriction fragment length polymorphism (PCR-RFLP).ResultsPatients were significantly more exposed to pesticides compared with the control group (p=0.0004), and the heterozygote genotype associated to exposure to pesticides also prevailed in patients (p=0.0001). Wild homozygote genotype was related to tobacco use (p=0.043) and alcoholism (p=0.033) in familial PD patients.ConclusionExposure to pesticides is associated to PD, whose effect can be enhanced when combined with the heterozygote genotype of GSTP1-Alw26I. Also, large genetic and environmental studies considering tobacco use, alcoholism, GSTP1 and PD are necessary to confirm our findings. ObjetivoAnalisar a frequência do polimorfismo GSTP1-Alw26I, assim como estimar sua associação com substâncias tóxicas na doença de Parkinson (DP).MétodosA casuística avaliada foi composta por um grupo de estudo, com 154 pacientes, subdivididos em DP familial e esporádica, e outro com 158 idosos sem a doença (grupo controle). O polimorfismo GSTP1-Alw26I foi analisado por reação em cadeia da polimerase/polimorfismo de comprimento do fragmento de restrição (PCR/RFLP).ResultadosOs pacientes foram significativamente mais expostos a pesticidas, comparados com o grupo controle (p=0,0004), e o genótipo heterozigoto associado a exposição a pesticidas também prevaleceu nos pacientes (p=0,0001). O genótipo homozigoto selvagem apresentou relação com tabagismo (p=0,043) e etilismo (p=0,033) em pacientes com DP familial. Desse modo, a exposição a pesticidas está associada à DP, cujo efeito pode ser potencializado quando combinado ao genótipo heterozigoto de GSTP1-Alw26I. Estudos genético-ambientais envolvendo tabagismo, etilismo, GSTP1 e DP devem ser realizados em casuísticas numerosas, confirmando essa associação.
Databáze: OpenAIRE
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