Inhibition of flower formation by antisense repression of mitochondrial citrate synthase in transgenic potato plants leads to a specific disintegration of the ovary tissues of flowers
| Autor: | Bernd Müller-Röber, L. Willmitzer, Volker Landschütze |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
Citrate (si)-Synthase
General Immunology and Microbiology biology General Neuroscience Transgene fungi Ovary (botany) food and beverages Genetically modified crops Plants Genetically Modified DNA Mitochondrial General Biochemistry Genetics and Molecular Biology Antisense RNA Mitochondria Citric acid cycle Biochemistry biology.protein Citrate synthase RNA Antisense Molecular Biology Flower formation Research Article Solanum tuberosum |
| Popis: | The tricarboxylic acid (TCA) cycle constitutes a major component of the mitochondrial metabolism of eucaryotes, including higher plants. To analyze the importance of this pathway, we down-regulated mitochondrial citrate synthase (mCS; EC 4.1.3.7), the first enzyme of the TCA cycle, in transgenic potato plants using an antisense RNA approach. Several transformants were identified with reduced citrate synthase activity (down to approximately 6% of wild-type activity). These plants were indistinguishable from wild-type plants in the greenhouse during vegetative growth. A major change, however, was seen upon initiation of the generative phase (flower formation). In the case of transgenic plants with a strong reduction in citrate synthase activity (< 30% of wild-type levels), flower buds formed > 2 weeks later as compared with wild-type plants. Furthermore, flower buds from these plants did not develop into mature flowers but rather were aborted at an early stage of development. Microscopic analysis showed that in these cases ovaries disintegrated during flower development. We conclude that the TCA cycle is of major importance during the transition from the vegetative to the generative phase. |
| Databáze: | OpenAIRE |
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