Microbial diversity and hydrocarbon degrading gene capacity of a crude oil field soil as determined by metagenomics analysis
| Autor: | Thavamani Palanisami, Mallavarapu Megharaj, Kavitha Ramadass, Firouz Abbasian, Ravi Naidu, Robin Lockington |
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| Přispěvatelé: | Abbasian, Firouz, Palanisami, Thavamani, Megharaj, Mallavarapu, Naidu, Ravi, Lockington, Robin, Ramadass, Kavitha |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microorganism aromatic hydrocarbons 010501 environmental sciences 01 natural sciences Actinobacteria Soil 03 medical and health sciences chemistry.chemical_compound Bioremediation Actinomycetales Food science Illumina dye sequencing Alphaproteobacteria 0105 earth and related environmental sciences chemistry.chemical_classification biology business.industry crude oil well Biodegradation biology.organism_classification Soil contamination Hydrocarbons Biotechnology Petroleum 030104 developmental biology Hydrocarbon chemistry microbial diversity alkanes Metagenomics business metagenomic study |
| Zdroj: | Biotechnology Progress. 32:638-648 |
| ISSN: | 8756-7938 |
| Popis: | Soils contaminated with crude oil are rich sources of enzymes suitable for both degradation of hydrocarbons through bioremediation processes and improvement of crude oil during its refining steps. Due to the long term selection, crude oil fields are unique environments for the identification of microorganisms with the ability to produce these enzymes. In this metagenomic study, based on Hiseq Illumina sequencing of samples obtained from a crude oil field and analysis of data on MG-RAST, Actinomycetales (9.8%) were found to be the dominant microorganisms, followed by Rhizobiales (3.3%). Furthermore, several functional genes were found in this study, mostly belong to Actinobacteria (12.35%), which have a role in the metabolism of aliphatic and aromatic hydrocarbons (2.51%), desulfurization (0.03%), element shortage (5.6%), and resistance to heavy metals (1.1%). This information will be useful for assisting in the application of microorganisms in the removal of hydrocarbon contamination and/or for improving the quality of crude oil. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:638-648, 2016. |
| Databáze: | OpenAIRE |
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